A Library of Induced Pluripotent Stem Cells from Clinically Well-characterized, Diverse Healthy Human Individuals

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2021 Nov 5

PMID 34739849

Citations 13

Authors

Christoph Schaniel

Priyanka Dhanan

Bin Hu

Yuguang Xiong

Teeya Raghunandan

David M Gonzalez

Rafael Dariolli

Sunita L DSouza

Arjun S Yadaw

Jens Hansen

Gomathi Jayaraman

Bino Mathew

Moara Machado

Seth I Berger

Joseph Tripodi

Vesna Najfeld

Jalaj Garg

Marc Miller

Colleen S Surlyn

Katherine C Michelis

Neelima C Tangirala

Himali Weerahandi

David C Thomas

Kristin G Beaumont

Robert Sebra

Milind Mahajan

Eric Schadt

Dusica Vidovic

Stephan C Schurer

Joseph Goldfarb

Evren U Azeloglu

Marc R Birtwistle

Eric A Sobie

Jason C Kovacic

Nicole C Dubois

Ravi Iyengar

Affiliations

Soon will be listed here.

Abstract

A library of well-characterized human induced pluripotent stem cell (hiPSC) lines from clinically healthy human subjects could serve as a useful resource of normal controls for in vitro human development, disease modeling, genotype-phenotype association studies, and drug response evaluation. We report generation and extensive characterization of a gender-balanced, racially/ethnically diverse library of hiPSC lines from 40 clinically healthy human individuals who range in age from 22 to 61 years. The hiPSCs match the karyotype and short tandem repeat identities of their parental fibroblasts, and have a transcription profile characteristic of pluripotent stem cells. We provide whole-genome sequencing data for one hiPSC clone from each individual, genomic ancestry determination, and analysis of mendelian disease genes and risks. We document similar transcriptomic profiles, single-cell RNA-sequencing-derived cell clusters, and physiology of cardiomyocytes differentiated from multiple independent hiPSC lines. This extensive characterization makes this hiPSC library a valuable resource for many studies on human biology.

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