Insertion/deletion and Microsatellite Alteration Profiles in Induced Pluripotent Stem Cells

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2021 Sep 24

PMID 34559999

Citations 2

Authors

Satoshi Kamimura

Tomo Suga

Yuko Hoki

Misato Sunayama

Kaori Imadome

Mayumi Fujita

Miki Nakamura

Ryoko Araki

Masumi Abe

Affiliations

Soon will be listed here.

Abstract

We here demonstrate that microsatellite (MS) alterations are elevated in both mouse and human induced pluripotent stem cells (iPSCs), but importantly we have now identified a type of human iPSC in which these alterations are considerably reduced. We aimed in our present analyses to profile the InDels in iPSC/ntESC genomes, especially in MS regions. To detect somatic de novo mutations in particular, we generated 13 independent reprogramed stem cell lines (11 iPSC and 2 ntESC lines) from an identical parent somatic cell fraction of a C57BL/6 mouse. By using this cell set with an identical genetic background, we could comprehensively detect clone-specific alterations and, importantly, experimentally validate them. The effectiveness of employing sister clones for detecting somatic de novo mutations was thereby demonstrated. We then successfully applied this approach to human iPSCs. Our results require further careful genomic analysis but make an important inroad into solving the issue of genome abnormalities in iPSCs.

Citing Articles

iPS cell generation-associated point mutations include many C > T substitutions via different cytosine modification mechanisms.

Araki R, Suga T, Hoki Y, Imadome K, Sunayama M, Kamimura S Nat Commun. 2024; 15(1):4946.

PMID: 38862540 PMC: 11166658. DOI: 10.1038/s41467-024-49335-5.

HLA-based banking of induced pluripotent stem cells in Saudi Arabia.

Alowaysi M, Lehmann R, Al-Shehri M, Baadhaim M, Alzahrani H, Aboalola D Stem Cell Res Ther. 2023; 14(1):374.

PMID: 38111036 PMC: 10729375. DOI: 10.1186/s13287-023-03612-0.

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