Induced Pluripotent Stem Cell Generation-associated Point Mutations Arise During the Initial Stages of the Conversion of These Cells

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2014 Feb 11

PMID 24511470

Citations 34

Authors

Mayumi Sugiura

Yasuji Kasama

Ryoko Araki

Yuko Hoki

Misato Sunayama

Masahiro Uda

Miki Nakamura

Shunsuke Ando

Masumi Abe

Affiliations

Soon will be listed here.

Abstract

A large number of point mutations have been identified in induced pluripotent stem cell (iPSC) genomes to date. Whether these mutations are associated with iPSC generation is an important and controversial issue. In this study, we approached this critical issue in different ways, including an assessment of iPSCs versus embryonic stem cells (ESCs), and an investigation of variant allele frequencies and the heterogeneity of point mutations within a single iPSC clone. Through these analyses, we obtained strong evidence that iPSC-generation-associated point mutations occur frequently in a transversion-predominant manner just after the onset of cell lineage conversion. The heterogeneity of the point mutation profiles within an iPSC clone was also revealed and reflects the history of the emergence of each mutation. Further, our results suggest a possible approach for establishing iPSCs with fewer point mutations.

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