Ajuba Transactivates N-cadherin Expression in Colorectal Cancer Cells Through Interaction with Twist

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2021 Jun 26

PMID 34173718

Citations 6

Authors

Zhaoxia Wu

Xiuqun Zou

Ying Xu

Fengli Zhou

Rong Kuai

Ji Li

Daming Yang

Yimin Chu

Haixia Peng

Affiliations

Soon will be listed here.

Abstract

Ajuba is a multiple LIM domain-containing protein and functions as a transcriptional coregulator to modulate many gene expressions in various cellular processes. Here, we describe that the LIM domain of Ajuba interacts with Twist, and the Twist box is a pivotal motif for the interaction. Biologically, Ajuba enhances transcription of target gene N-cadherin as an obligate coactivator of Twist. The enhancement is achieved by binding to the E-box element within N-cadherin promoter as revealed by luciferase reporter and chromatin immunoprecipitation assays. Mechanistic investigation demonstrates that Ajuba recruits CBP and Twist to form a ternary complex at the Twist target promoter region and concomitantly enhances histone acetylation at these sites. These findings identify that Twist is a new interacting protein of Ajuba and Ajuba/Twist/CBP ternary complex may be a potential treatment strategy for Twist-related tumour metastasis.

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