14-3-3 Binding Sites in the Snail Protein Are Essential for Snail-mediated Transcriptional Repression and Epithelial-mesenchymal Differentiation

Overview

Journal Cancer Res

Specialty Oncology

Date 2010 May 27

PMID 20501852

Citations 47

Authors

Zhaoyuan Hou

Hongzhuang Peng

David E White

Pu Wang

Paul M Lieberman

Thanos Halazonetis

Frank J Rauscher 3rd

Affiliations

Soon will be listed here.

Abstract

The Snail transcription factor is a repressor and a master regulator of epithelial-mesenchymal transition (EMT) events in normal embryonic development and during tumor metastases. Snail directly regulates genes affecting cell adhesion, motility, and polarity. Invasive tumor cells express high levels of Snail, which is a marker for aggressive disease and poor prognosis. Transcriptional repression and EMT induction by Snail requires binding to its obligate corepressor, the LIM protein Ajuba. It is unclear how this complex is assembled and maintained on Snail target genes. Here we define functional 14-3-3 binding motifs in Snail and Ajuba, which selectively bind 14-3-3 protein isoforms. In Snail, an NH(2)-terminal motif in the repression domain cooperates with a COOH-terminal, high-affinity motif for binding to 14-3-3 proteins. Coordinate mutation of both motifs abolishes 14-3-3 binding and inhibits Snail-mediated gene repression and EMT differentiation. Snail, 14-3-3 proteins, and Ajuba form a ternary complex that is readily detected through chromatin immunoprecipitation at the endogenous E-cadherin promoter. Collectively, these data show that 14-3-3 proteins are new components of the Snail transcriptional repression machinery and mediate its important biological functions.

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