Extract of R. Prevents Atherosclerosis in Apolipoprotein E-deficient Mice

Overview

Journal Nutr Res Pract

Specialty Nutritional Sciences

Date 2021 Jun 7

PMID 34093973

Authors

Ki Mo Kim

Joo Young Lee

Byeong Hwa Jeon

Khong Trong Quan

MinKyun Na

Kung-Woo Nam

Sungwook Chae

Affiliations

Soon will be listed here.

Abstract

Background/objectives: R. () has been used to treat headache, fever, and hypertension-related symptoms in Asian countries, including Korea, China, and Japan. We investigated whether dietary intake of a extract (CzE) affected atherosclerosis .

Materials/methods: Apolipoprotein E-deficient (ApoE) mice (n = 32) were fed a normal diet (ND), a high-cholesterol diet (HCD), an HCD containing CzE (100 mg/kg/day), or an HCD containing simvastatin (10 mg/kg/day) for 12 weeks. The anti-atherosclerotic effects were evaluated by observing changes in fatty streak lesions, immunohistochemical analysis, fluorescence imaging, lipid profiles, and western blot analysis.

Results: The CzE-fed group showed a 41.6% reduction of atherosclerosis. Furthermore, CzE significantly reduced the levels of serum triglyceride, high-density lipoprotein, the chemokine (C-X3-C-motif) ligand 1, the adhesion molecules vascular cell adhesion molecule-1, intracellular adhesion molecule-1, and E-selectin; down-regulation of tumor necrosis factor-α, interleukin-6, high mobility group box-1, and cathepsin levels in the aortic sinuses and aortas of ApoE mice were also observed.

Conclusions: The results suggest that the inclusion of a water extract of in a HCD is closely correlated with reducing the risk of vascular inflammatory diseases in an ApoE mouse model.

References

Hamdi O, Feroz S, Shilpi J, Anouar E, Mukarram A, Mohamad S . Spectrofluorometric and molecular docking studies on the binding of curcumenol and curcumenone to human serum albumin. Int J Mol Sci. 2015; 16(3):5180-93. PMC: 4394470. DOI: 10.3390/ijms16035180. View

Akter J, Hossain M, Takara K, Islam M, Hou D . Antioxidant activity of different species and varieties of turmeric (Curcuma spp): Isolation of active compounds. Comp Biochem Physiol C Toxicol Pharmacol. 2018; 215:9-17. DOI: 10.1016/j.cbpc.2018.09.002. View

Lee H, Im A, Kim S, Kang H, Lee J, Chae S . The flavonoid hesperidin exerts anti-photoaging effect by downregulating matrix metalloproteinase (MMP)-9 expression via mitogen activated protein kinase (MAPK)-dependent signaling pathways. BMC Complement Altern Med. 2018; 18(1):39. PMC: 5791357. DOI: 10.1186/s12906-017-2058-8. View

Yao C, Jiang J, Tu Y, Ye S, Du H, Zhang Y . β-elemene reverses the drug resistance of A549/DDP lung cancer cells by activating intracellular redox system, decreasing mitochondrial membrane potential and P-glycoprotein expression, and inducing apoptosis. Thorac Cancer. 2016; 5(4):304-12. PMC: 4704358. DOI: 10.1111/1759-7714.12093. View

Barlic J, Zhang Y, Murphy P . Atherogenic lipids induce adhesion of human coronary artery smooth muscle cells to macrophages by up-regulating chemokine CX3CL1 on smooth muscle cells in a TNFalpha-NFkappaB-dependent manner. J Biol Chem. 2007; 282(26):19167-76. DOI: 10.1074/jbc.M701642200. View

Libby P . Inflammation in atherosclerosis. Nature. 2002; 420(6917):868-74. DOI: 10.1038/nature01323. View

Lee T, Lee D, Lee S, Ko Y, Kang K, Chung S . Sesquiterpenes from Curcuma zedoaria rhizomes and their cytotoxicity against human gastric cancer AGS cells. Bioorg Chem. 2019; 87:117-122. DOI: 10.1016/j.bioorg.2019.03.015. View

Zhang S, Reddick R, Piedrahita J, Maeda N . Spontaneous hypercholesterolemia and arterial lesions in mice lacking apolipoprotein E. Science. 1992; 258(5081):468-71. DOI: 10.1126/science.1411543. View

Su Q, Sun Y, Ye Z, Yang H, Kong B, Li L . Pinocembrin protects endothelial cells from oxidized LDL-induced injury. Cytokine. 2018; 111:475-480. DOI: 10.1016/j.cyto.2018.05.033. View

10.

Liu J, Sukhova G, Sun J, Xu W, Libby P, Shi G . Lysosomal cysteine proteases in atherosclerosis. Arterioscler Thromb Vasc Biol. 2004; 24(8):1359-66. DOI: 10.1161/01.ATV.0000134530.27208.41. View

11.

Musumeci D, Roviello G, Montesarchio D . An overview on HMGB1 inhibitors as potential therapeutic agents in HMGB1-related pathologies. Pharmacol Ther. 2013; 141(3):347-57. DOI: 10.1016/j.pharmthera.2013.11.001. View

12.

Wu H, Du Q, Dai Q, Ge J, Cheng X . Cysteine Protease Cathepsins in Atherosclerotic Cardiovascular Diseases. J Atheroscler Thromb. 2017; 25(2):111-123. PMC: 5827079. DOI: 10.5551/jat.RV17016. View

13.

Im A, Yang W, Park Y, Kim S, Chae S . Hepatoprotective Effects of Insect Extracts in an Animal Model of Nonalcoholic Fatty Liver Disease. Nutrients. 2018; 10(6). PMC: 6024666. DOI: 10.3390/nu10060735. View

14.

Paigen B, Morrow A, Holmes P, Mitchell D, Williams R . Quantitative assessment of atherosclerotic lesions in mice. Atherosclerosis. 1987; 68(3):231-40. DOI: 10.1016/0021-9150(87)90202-4. View

15.

Le T, Beaufay C, Nghiem D, Pham T, Mingeot-Leclercq M, Quetin-Leclercq J . Evaluation of the Anti-Trypanosomal Activity of Vietnamese Essential Oils, with Emphasis on L. and Its Components. Molecules. 2019; 24(6). PMC: 6471621. DOI: 10.3390/molecules24061158. View

16.

Navarro D, de Souza M, Neto R, Golin V, Niero R, Yunes R . Phytochemical analysis and analgesic properties of Curcuma zedoaria grown in Brazil. Phytomedicine. 2002; 9(5):427-32. DOI: 10.1078/09447110260571670. View

17.

Jaffer F, Libby P, Weissleder R . Molecular imaging of cardiovascular disease. Circulation. 2007; 116(9):1052-61. DOI: 10.1161/CIRCULATIONAHA.106.647164. View

18.

Jung E, Trinh T, Lee T, Yamabe N, Kang K, Song J . Curcuzedoalide contributes to the cytotoxicity of Curcuma zedoaria rhizomes against human gastric cancer AGS cells through induction of apoptosis. J Ethnopharmacol. 2017; 213:48-55. DOI: 10.1016/j.jep.2017.10.025. View

19.

Lee S, Kim S, Oh O, Min H, Park K, Chung W . Suppressive effect of natural sesquiterpenoids on inducible cyclooxygenase (COX-2) and nitric oxide synthase (iNOS) activity in mouse macrophage cells. J Environ Pathol Toxicol Oncol. 2002; 21(2):141-8. View

20.

Makabe H, Maru N, Kuwabara A, Kamo T, Hirota M . Anti-inflammatory sesquiterpenes from Curcuma zedoaria. Nat Prod Res. 2006; 20(7):680-5. DOI: 10.1080/14786410500462900. View