Baicalein Alleviates Osteoarthritis by Protecting Subchondral Bone, Inhibiting Angiogenesis and Synovial Proliferation

Overview

Journal J Cell Mol Med

Specialties Cell Biology
Molecular Biology

Date 2021 May 3

PMID 33939310

Citations 20

Authors

Bin Li

Kaizhe Chen

Niandong Qian

Ping Huang

Fangqiong Hu

Tao Ding

Xing Xu

Qi Zhou

Bo Chen

Lianfu Deng

Tianwen Ye

Lei Guo

Affiliations

Soon will be listed here.

Abstract

Osteoarthritis (OA) is one of the most frequent chronic joint diseases with the increasing life expectancy. The main characteristics of the disease are loss of articular cartilage, subchondral bone sclerosis and synovium inflammation. Physical measures, drug therapy and surgery are the mainstay of treatments for OA, whereas drug therapies are mainly limited to analgesics, glucocorticoids, hyaluronic acids and some alternative therapies because of single therapeutic target of OA joints. Baicalein, a traditional Chinese medicine extracted from Scutellaria baicalensis Georgi, has been widely used in anti-inflammatory therapies. Previous studies revealed that baicalein could alleviate cartilage degeneration effectively by acting on articular chondrocytes. However, the mechanisms involved in baicalein-mediated protection of the OA are not completely understood in consideration of integrality of arthrosis. In this study, we found that intra-articular injection of baicalein ameliorated subchondral bone remodelling. Further studies showed that baicalein could decrease the number of differentiated osteoblasts by inhibiting pre-osteoblasts proliferation and promoting pre-osteoblasts apoptosis. In addition, baicalein impaired angiogenesis of endothelial cells and inhibited proliferation of synovial cells. Taken together, these results implicated that baicalein might be an effective medicine for treating OA by regulating multiple targets.

Citing Articles

A Baicalin-Based Functional Polymer in Dynamic Reversible Networks Alleviates Osteoarthritis by Cellular Interactions.

Yang Y, Hu Q, Shao Q, Peng Y, Yu B, Luo F Adv Sci (Weinh). 2025; 12(10):e2410951.

PMID: 39840483 PMC: 11904974. DOI: 10.1002/advs.202410951.

Effects of Baicalein Pretreatment on the NLRP3/GSDMD Pyroptosis Pathway and Neuronal Injury in Pilocarpine-Induced Status Epilepticus in the Mice.

Kang J, Mo S, Shu X, Cheng S eNeuro. 2024; 12(1.

PMID: 39662962 PMC: 11728850. DOI: 10.1523/ENEURO.0319-24.2024.

Baicalein suppresses inflammation and attenuates acute lung injury by inhibiting glycolysis via HIF‑1α signaling.

Liu Z, Zheng X, Li N, Wang Z Mol Med Rep. 2024; 31(1).

PMID: 39513601 PMC: 11564906. DOI: 10.3892/mmr.2024.13383.

Novel formulations ameliorate osteoarthritis in rats by inhibiting inflammation and oxidative stress.

Erten F, Ozdemir O, Tokmak M, Durmus A, Ozercan I, Morde A Food Sci Nutr. 2024; 12(10):7896-7912.

PMID: 39479600 PMC: 11521736. DOI: 10.1002/fsn3.4407.

Amentoflavone maintaining extracellular matrix homeostasis and inhibiting subchondral bone loss in osteoarthritis by inhibiting ERK, JNK and NF-κB signaling pathways.

Liang J, Hu J, Hong X, Zhou M, Xia G, Hu L J Orthop Surg Res. 2024; 19(1):662.

PMID: 39407273 PMC: 11481797. DOI: 10.1186/s13018-024-05075-2.

References

Henrotin Y, Pesesse L, Sanchez C . Subchondral bone and osteoarthritis: biological and cellular aspects. Osteoporos Int. 2012; 23 Suppl 8:S847-51. DOI: 10.1007/s00198-012-2162-z. View

Bie B, Sun J, Guo Y, Li J, Jiang W, Yang J . Baicalein: A review of its anti-cancer effects and mechanisms in Hepatocellular Carcinoma. Biomed Pharmacother. 2017; 93:1285-1291. DOI: 10.1016/j.biopha.2017.07.068. View

Park Y, Choi J, Jung H, Kim B, Kim C, Park S . Baicalein inhibits tumor progression by inhibiting tumor cell growth and tumor angiogenesis. Oncol Rep. 2017; 38(5):3011-3018. DOI: 10.3892/or.2017.6007. View

Zhang X, Zhu Y, Chen X, Zhang Y, Zhang Y, Jia Y . Baicalein ameliorates inflammatory-related apoptotic and catabolic phenotypes in human chondrocytes. Int Immunopharmacol. 2014; 21(2):301-8. DOI: 10.1016/j.intimp.2014.05.006. View

Shi C, Huang P, Kang H, Hu B, Qi J, Jiang M . Glucocorticoid inhibits cell proliferation in differentiating osteoblasts by microRNA-199a targeting of WNT signaling. J Mol Endocrinol. 2015; 54(3):325-37. DOI: 10.1530/JME-14-0314. View

Armiento A, Stoddart M, Alini M, Eglin D . Biomaterials for articular cartilage tissue engineering: Learning from biology. Acta Biomater. 2017; 65:1-20. DOI: 10.1016/j.actbio.2017.11.021. View

Chen W, Xiong Y, Hu P, Bao J, Wu L . Baicalein Inhibits MMPs Expression via a MAPK-Dependent Mechanism in Chondrocytes. Cell Physiol Biochem. 2015; 36(1):325-33. DOI: 10.1159/000374075. View

Xu T, Ge X, Lu C, Dai W, Chen H, Xiao Z . Baicalein attenuates OVA-induced allergic airway inflammation through the inhibition of the NF-κB signaling pathway. Aging (Albany NY). 2019; 11(21):9310-9327. PMC: 6874438. DOI: 10.18632/aging.102371. View

Muraoka T, Hagino H, Okano T, Enokida M, Teshima R . Role of subchondral bone in osteoarthritis development: a comparative study of two strains of guinea pigs with and without spontaneously occurring osteoarthritis. Arthritis Rheum. 2007; 56(10):3366-74. DOI: 10.1002/art.22921. View

10.

Zhao W, Wang T, Luo Q, Chen Y, Leung V, Wen C . Cartilage degeneration and excessive subchondral bone formation in spontaneous osteoarthritis involves altered TGF-β signaling. J Orthop Res. 2015; 34(5):763-70. DOI: 10.1002/jor.23079. View

11.

Lu J, Zhang H, Cai D, Zeng C, Lai P, Shao Y . Positive-Feedback Regulation of Subchondral H-Type Vessel Formation by Chondrocyte Promotes Osteoarthritis Development in Mice. J Bone Miner Res. 2018; 33(5):909-920. DOI: 10.1002/jbmr.3388. View

12.

Li Y, Yang B, Bai J, Xia S, Mao M, Li X . The roles of synovial hyperplasia, angiogenesis and osteoclastogenesis in the protective effect of apigenin on collagen-induced arthritis. Int Immunopharmacol. 2019; 73:362-369. DOI: 10.1016/j.intimp.2019.05.024. View

13.

Haywood L, McWilliams D, Pearson C, Gill S, Ganesan A, Wilson D . Inflammation and angiogenesis in osteoarthritis. Arthritis Rheum. 2003; 48(8):2173-7. DOI: 10.1002/art.11094. View

14.

Pesesse L, Sanchez C, Henrotin Y . Osteochondral plate angiogenesis: a new treatment target in osteoarthritis. Joint Bone Spine. 2010; 78(2):144-9. DOI: 10.1016/j.jbspin.2010.07.001. View

15.

Chen D, Shen J, Zhao W, Wang T, Han L, Hamilton J . Osteoarthritis: toward a comprehensive understanding of pathological mechanism. Bone Res. 2017; 5:16044. PMC: 5240031. DOI: 10.1038/boneres.2016.44. View

16.

Li B, Chen K, Qian N, Huang P, Hu F, Ding T . Baicalein alleviates osteoarthritis by protecting subchondral bone, inhibiting angiogenesis and synovial proliferation. J Cell Mol Med. 2021; 25(11):5283-5294. PMC: 8178278. DOI: 10.1111/jcmm.16538. View

17.

Yu D, Xu J, Liu F, Wang X, Mao Y, Zhu Z . Subchondral bone changes and the impacts on joint pain and articular cartilage degeneration in osteoarthritis. Clin Exp Rheumatol. 2016; 34(5):929-934. View

18.

Cui Z, Crane J, Xie H, Jin X, Zhen G, Li C . Halofuginone attenuates osteoarthritis by inhibition of TGF-β activity and H-type vessel formation in subchondral bone. Ann Rheum Dis. 2015; 75(9):1714-21. PMC: 5013081. DOI: 10.1136/annrheumdis-2015-207923. View

19.

Amin M, Fox D, Ruth J . Synovial cellular and molecular markers in rheumatoid arthritis. Semin Immunopathol. 2017; 39(4):385-393. PMC: 5521018. DOI: 10.1007/s00281-017-0631-3. View

20.

Bi X, Guo X, Mo B, Wang M, Luo X, Chen Y . LncRNA PICSAR promotes cell proliferation, migration and invasion of fibroblast-like synoviocytes by sponging miRNA-4701-5p in rheumatoid arthritis. EBioMedicine. 2019; 50:408-420. PMC: 6921299. DOI: 10.1016/j.ebiom.2019.11.024. View