Dual-Viral Transduction Utilizing Highly Efficient Retrograde Lentivirus Improves Labeling of Long Propriospinal Neurons

Overview

Journal Front Neuroanat

Date 2021 Apr 8

PMID 33828464

Citations 2

Authors

Brandon L Brown

Rachel M Zalla

Courtney T Shepard

Russell M Howard

Jonathan A Kopechek

David S K Magnuson

Scott R Whittemore

Affiliations

Soon will be listed here.

Abstract

The nervous system coordinates pathways and circuits to process sensory information and govern motor behaviors. Mapping these pathways is important to further understand the connectivity throughout the nervous system and is vital for developing treatments for neuronal diseases and disorders. We targeted long ascending propriospinal neurons (LAPNs) in the rat spinal cord utilizing Fluoro-Ruby (FR) [10kD rhodamine dextran amine (RDA)], and two dual-viral systems. Dual-viral tracing utilizing a retrograde adeno-associated virus (retroAAV), which confers robust labeling in the brain, resulted in a small number of LAPNs being labeled, but dual-viral tracing using a highly efficient retrograde (HiRet) lentivirus provided robust labeling similar to FR. Additionally, dual-viral tracing with HiRet lentivirus and tracing with FR may preferentially label different subpopulations of LAPNs. These data demonstrate that dual-viral tracing in the spinal cord employing a HiRet lentivirus provides robust and specific labeling of LAPNs and emphasizes the need to empirically optimize viral systems to target specific neuronal population(s).

Citing Articles

Long ascending propriospinal neurons are heterogenous and subject to spinal cord injury induced anatomic plasticity.

Brown B, Anil N, States G, Whittemore S, Magnuson D Exp Neurol. 2023; 373:114631.

PMID: 38070723 PMC: 10922963. DOI: 10.1016/j.expneurol.2023.114631.

Silencing long ascending propriospinal neurons after spinal cord injury improves hindlimb stepping in the adult rat.

Shepard C, Pocratsky A, Brown B, Van Rijswijck M, Zalla R, Burke D Elife. 2021; 10.

PMID: 34854375 PMC: 8639151. DOI: 10.7554/eLife.70058.

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