IgE-Reactivity Pattern of Tomato Seed and Peel Nonspecific Lipid-Transfer Proteins After Gastrointestinal Digestion

Overview

Journal J Agric Food Chem

Specialties Chemistry
Nutritional Sciences

Date 2021 Mar 15

PMID 33719421

Citations 3

Authors

Laura Martin-Pedraza

Cristobalina Mayorga

Francisca Gomez

Cristina Bueno-Diaz

Natalia Blanca-Lopez

Miguel Gonzalez

Monica Martinez-Blanco

Javier Cuesta-Herranz

Elena Molina

Mayte Villalba

Sara Benede

Affiliations

Soon will be listed here.

Abstract

The influence of gastrointestinal digestion on the immunological properties of three different nonspecific lipid-transfer proteins (nsLTPs) described in tomato fruit has been assessed using an system mimicking the stomach and intestine digestion conditions. Tomato peel/pulp nsLTP, Sola l 3, was degraded after digestion, although the immunoglobulin E (IgE) recognition of intact protein and a 10 kDa band were still observed after 30 min of duodenal digestion in the presence of phosphatidylcholine. The tomato seed nsLTP, Sola l 7, showed a higher stability than the other seed allergen, Sola l 6, during digestion. Sola l 7 showed an IgE immunoreactive 6.5 kDa band in immunoblotting analysis, retaining up to 7% of its IgE-binding capacity in inhibition ELISA test after 60 min of duodenal digestion and keeping intact its ability to activate basophils after digestion. These results suggest that the tomato seed allergen Sola l 7 might be considered as an important allergen in the induction of allergic responses to tomato due to its high stability against gastrointestinal digestion.

Citing Articles

Long-chain fatty acids block allergic reaction against lipid transfer protein Sola l 7 from tomato seeds.

Parron-Ballesteros J, Martin-Pedraza L, Gordo R, Mayorga C, Pastor-Vargas C, Titaux-Delgado G Protein Sci. 2024; 33(9):e5154.

PMID: 39180496 PMC: 11344279. DOI: 10.1002/pro.5154.

Precision engineering for localization, validation, and modification of allergenic epitopes.

Pomes A, Smith S, Chruszcz M, Mueller G, Brackett N, Chapman M J Allergy Clin Immunol. 2024; 153(3):560-571.

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LTP Allergy Follow-Up Study: Development of Allergy to New Plant Foods 10 Years Later.

Betancor D, Gomez-Lopez A, Villalobos-Vilda C, Nunez-Borque E, Fernandez-Bravo S, De Las Heras Gozalo M Nutrients. 2021; 13(7).

PMID: 34202484 PMC: 8308245. DOI: 10.3390/nu13072165.

References

Fleury C, Gracy J, Gautier M, Pons J, Dufayard J, Labesse G . Comprehensive classification of the plant non-specific lipid transfer protein superfamily towards its sequence-structure-function analysis. PeerJ. 2019; 7:e7504. PMC: 6698131. DOI: 10.7717/peerj.7504. View

Van Ree R . Clinical importance of non-specific lipid transfer proteins as food allergens. Biochem Soc Trans. 2002; 30(Pt 6):910-3. DOI: 10.1042/bst0300910. View

Jimenez-Saiz R, Benede S, Miralles B, Lopez-Exposito I, Molina E, Lopez-Fandino R . Immunological behavior of in vitro digested egg-white lysozyme. Mol Nutr Food Res. 2013; 58(3):614-24. DOI: 10.1002/mnfr.201300442. View

Sirvent S, Canto B, Gomez F, Blanca N, Cuesta-Herranz J, Canto G . Detailed characterization of Act d 12 and Act d 13 from kiwi seeds: implication in IgE cross-reactivity with peanut and tree nuts. Allergy. 2014; 69(11):1481-8. DOI: 10.1111/all.12486. View

Lozano-Ojalvo D, Lopez-Fandino R . Immunomodulating peptides for food allergy prevention and treatment. Crit Rev Food Sci Nutr. 2017; 58(10):1629-1649. DOI: 10.1080/10408398.2016.1275519. View

Berecz B, Mills E, Paradi I, Lang F, Tamas L, Shewry P . Stability of sunflower 2S albumins and LTP to physiologically relevant in vitro gastrointestinal digestion. Food Chem. 2013; 138(4):2374-81. DOI: 10.1016/j.foodchem.2012.12.034. View

Bogh K, Madsen C . Food Allergens: Is There a Correlation between Stability to Digestion and Allergenicity?. Crit Rev Food Sci Nutr. 2015; 56(9):1545-67. DOI: 10.1080/10408398.2013.779569. View

Sicherer S, Sampson H . Food allergy: A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management. J Allergy Clin Immunol. 2017; 141(1):41-58. DOI: 10.1016/j.jaci.2017.11.003. View

Westphal S, Kempf W, Foetisch K, Retzek M, Vieths S, Scheurer S . Tomato profilin Lyc e 1: IgE cross-reactivity and allergenic potency. Allergy. 2004; 59(5):526-32. DOI: 10.1046/j.1398-9995.2003.00413.x. View

10.

Benede S, Lopez-Exposito I, Molina E, Lopez-Fandino R . Egg proteins as allergens and the effects of the food matrix and processing. Food Funct. 2015; 6(3):694-713. DOI: 10.1039/c4fo01104j. View

11.

Westphal S, Kolarich D, Foetisch K, Lauer I, Altmann F, Conti A . Molecular characterization and allergenic activity of Lyc e 2 (beta-fructofuranosidase), a glycosylated allergen of tomato. Eur J Biochem. 2003; 270(6):1327-37. DOI: 10.1046/j.1432-1033.2003.03503.x. View

12.

Heinzerling L, Mari A, Bergmann K, Bresciani M, Burbach G, Darsow U . The skin prick test - European standards. Clin Transl Allergy. 2013; 3(1):3. PMC: 3565910. DOI: 10.1186/2045-7022-3-3. View

13.

McKenna O, Asam C, Araujo G, Roulias A, Goulart L, Ferreira F . How relevant is panallergen sensitization in the development of allergies?. Pediatr Allergy Immunol. 2016; 27(6):560-8. PMC: 5006871. DOI: 10.1111/pai.12589. View

14.

Ballmer-Weber B, Hoffmann-Sommergruber K . Molecular diagnosis of fruit and vegetable allergy. Curr Opin Allergy Clin Immunol. 2011; 11(3):229-35. DOI: 10.1097/ACI.0b013e3283464c74. View

15.

Scheurer S, Lauer I, Foetisch K, Moncin M, Retzek M, Hartz C . Strong allergenicity of Pru av 3, the lipid transfer protein from cherry, is related to high stability against thermal processing and digestion. J Allergy Clin Immunol. 2004; 114(4):900-7. DOI: 10.1016/j.jaci.2004.06.017. View

16.

Untersmayr E, Jensen-Jarolim E . The role of protein digestibility and antacids on food allergy outcomes. J Allergy Clin Immunol. 2008; 121(6):1301-8. PMC: 2999748. DOI: 10.1016/j.jaci.2008.04.025. View

17.

Wangorsch A, Jamin A, Foetisch K, Malczyk A, Reuter A, Vierecke S . Identification of Sola l 4 as Bet v 1 homologous pathogenesis related-10 allergen in tomato fruits. Mol Nutr Food Res. 2014; 59(3):582-92. DOI: 10.1002/mnfr.201300620. View

18.

Samuel D, Liu Y, Cheng C, Lyu P . Solution structure of plant nonspecific lipid transfer protein-2 from rice (Oryza sativa). J Biol Chem. 2002; 277(38):35267-73. DOI: 10.1074/jbc.M203113200. View

19.

Lozano-Ojalvo D, Molina E, Lopez-Fandino R . Hydrolysates of egg white proteins modulate T- and B-cell responses in mitogen-stimulated murine cells. Food Funct. 2016; 7(2):1048-56. DOI: 10.1039/c5fo00614g. View

20.

Lindorff-Larsen K, Winther J . Surprisingly high stability of barley lipid transfer protein, LTP1, towards denaturant, heat and proteases. FEBS Lett. 2001; 488(3):145-8. DOI: 10.1016/s0014-5793(00)02424-8. View