Sequencing of the Arabidopsis NOR2 Reveals Its Distinct Organization and Tissue-specific RRNA Ribosomal Variants

Overview

Journal Nat Commun

Specialty Biology

Date 2021 Jan 16

PMID 33452254

Citations 24

Authors

Jason Sims

Giovanni Sestini

Christiane Elgert

Arndt von Haeseler

Peter Schlogelhofer

Affiliations

Soon will be listed here.

Abstract

Despite vast differences between organisms, some characteristics of their genomes are conserved, such as the nucleolus organizing region (NOR). The NOR is constituted of multiple, highly repetitive rDNA genes, encoding the catalytic ribosomal core RNAs which are transcribed from 45S rDNA units. Their precise sequence information and organization remain uncharacterized. Here, using a combination of long- and short-read sequencing technologies we assemble contigs of the Arabidopsis NOR2 rDNA domain. We identify several expressed rRNA gene variants which are integrated into translating ribosomes in a tissue-specific manner. These findings support the concept of tissue specific ribosome subpopulations that differ in their rRNA composition and provide insights into the higher order organization of NOR2.

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