An Arabidopsis FANCJ Helicase Homologue is Required for DNA Crosslink Repair and RDNA Repeat Stability

Overview

Journal PLoS Genet

Specialty Genetics

Date 2019 May 24

PMID 31120885

Citations 13

Authors

Annika Dorn

Laura Feller

Dominique Castri

Sarah Rohrig

Janina Enderle

Natalie J Herrmann

Astrid Block-Schmidt

Oliver Trapp

Laura Kohler

Holger Puchta

Affiliations

Soon will be listed here.

Abstract

Proteins of the Fanconi Anemia (FA) complementation group are required for crosslink (CL) repair in humans and their loss leads to severe pathological phenotypes. Here we characterize a homolog of the Fe-S cluster helicase FANCJ in the model plant Arabidopsis, AtFANCJB, and show that it is involved in interstrand CL repair. It acts at a presumably early step in concert with the nuclease FAN1 but independently of the nuclease AtMUS81, and is epistatic to both error-prone and error-free post-replicative repair in Arabidopsis. The simultaneous knock out of FANCJB and the Fe-S cluster helicase RTEL1 leads to induced cell death in root meristems, indicating an important role of the enzymes in replicative DNA repair. Surprisingly, we found that AtFANCJB is involved in safeguarding rDNA stability in plants. In the absence of AtRTEL1 and AtFANCJB, we detected a synergetic reduction to about one third of the original number of 45S rDNA copies. It is tempting to speculate that the detected rDNA instability might be due to deficiencies in G-quadruplex structure resolution and might thus contribute to pathological phenotypes of certain human genetic diseases.

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