A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2020 Dec 23

PMID 33352964

Authors

Yoshihisa Ito

Yusuke Izawa

Toshihisa Osaki

Koki Kamiya

Nobuo Misawa

Satoshi Fujii

Hisatoshi Mimura

Norihisa Miki

Shoji Takeuchi

Affiliations

Soon will be listed here.

Abstract

Lipid-bilayer devices have been studied for on-site sensors in the fields of diagnosis, food and environmental monitoring, and safety/security inspection. In this paper, we propose a lipid-bilayer-on-a-cup device for serial sample measurements using a pumpless solution exchange procedure. The device consists of a millimeter-scale cylindrical cup with vertical slits which is designed to steadily hold an aqueous solution and exchange the sample by simply fusing and splitting the solution with an external solution. The slit design was experimentally determined by the capabilities of both the retention and exchange of the solution. Using the optimized slit, a planar lipid bilayer was reconstituted with a nanopore protein at a microaperture allocated to the bottom of the cup, and the device was connected to a portable amplifier. The solution exchangeability was demonstrated by observing the dilution process of a blocker molecule of the nanopore dissolved in the cup. The pumpless solution exchange by the proposed cup-like device presents potential as a lipid-bilayer system for portable sensing applications.

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