Nanoparticles (PLGA and Chitosan)-Entrapped ADP-Ribosylation Factor 1 of Enhances the Immune Responses in ICR Mice

Overview

Journal Vaccines (Basel)

Date 2020 Dec 5

PMID 33276581

Citations 5

Authors

Muhammad Waqqas Hasan

Muhammad Haseeb

Muhammad Ehsan

Javaid Ali Gadahi

Muhammad Ali-Ul-Husnain Naqvi

Qiang Qiang Wang

Xinchao Liu

Shakeel Ahmed Lakho

Ruofeng Yan

Lixin Xu

Xiaokai Song

Xiangrui Li

Affiliations

Soon will be listed here.

Abstract

ADP-ribosylation factor 1 (HcARF1) is one of the () excretory/secretory proteins involved in modulating the immune response of goat peripheral blood mononuclear cells (PBMC). Here, we evaluated the immunogenic potential of recombinant HcARF1 (rHcARF1) against infection in Institute of Cancer Research (ICR) mice. Briefly, rHcARF1 was entrapped in poly (D, L-lactide-co-glycolide) (PLGA) and chitosan (CS) nanoparticles (NP) and injected into mice as a vaccine. Fifty-six ICR mice were assigned randomly into seven groups, with eight animals in each group, and they were vaccinated subcutaneously. At the end of the experiment (14th day), the blood and the spleen were collected from euthanized mice to detect lymphocyte proliferation, cytokine analysis, and the production of antigen-specific antibodies. Scanning electron microscope was used to determine the size, morphology, and zeta potential of nanoparticles. Flow cytometry was performed, which presented the increase percentages of CD4 T cells (CD3eCD4), CD8 T cells (CD3eCD8) and dendritic cells (CD11cCD83, CD11cCD86) in mice vaccinated with rHcARF1+PLGA NP. Immunoassay analysis show raised humoral (Immunoglobulin (Ig)G1, IgG2a, IgM) and cell-mediated immune response (Interleukin (IL)-4, IL-12, and IL-17, and Interferon (IFN)-γ) induced by rHcARF1+PLGA NP. Experimental groups that were treated with the antigen-loaded NP yield higher lymphocyte proliferation than the control groups. Based on these results, we could propose that the rHcARF1 encapsulated in NP could stimulate a strong immune response in mice rather than administering alone against the infection of .

Citing Articles

Nanoparticle containing recombinant excretory/secretory-24 protein of enhanced the cellular immune responses in mice.

Hasan M, Haseeb M, Gadahi J, Ehsan M, Wang Q, Lakho S Front Vet Sci. 2024; 11:1470084.

PMID: 39600880 PMC: 11588750. DOI: 10.3389/fvets.2024.1470084.

Partial Protection of Goats against Achieved with ADP-Ribosylation Factor 1 Encapsulated in PLGA Nanoparticles.

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PLGA-Encapsulated Antigen ES-15 Augments Immune Responses in a Murine Model.

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PMID: 38140198 PMC: 10748113. DOI: 10.3390/vaccines11121794.

Microfluidic-Based Formulation of Essential Oils-Loaded Chitosan Coated PLGA Particles Enhances Their Bioavailability and Nematocidal Activity.

Helal M, Abdel-Gawad A, Kandil O, Khalifa M, Morrison A, Bartley D Pharmaceutics. 2022; 14(10).

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Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of HCA59 Antigen in Model Mice.

Wang Q, Sun X, Huang X, Huang J, Hasan M, Yan R Int J Nanomedicine. 2021; 16:3125-3139.

PMID: 33981142 PMC: 8107376. DOI: 10.2147/IJN.S301851.

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