Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses of HCA59 Antigen in Model Mice

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2021 May 13

PMID 33981142

Citations 4

Authors

Qiangqiang Wang

Xiaoke Sun

Xin Huang

Jianmei Huang

Muhammad Waqqas Hasan

Ruofeng Yan

Lixin Xu

Xiaokai Song

Xiangrui Li

Affiliations

Soon will be listed here.

Abstract

Background: Hepatocellular carcinoma-associated antigen 59 (HCA59) from excretory/secretory products of is known to have the ability to modulate the functions of host cells. However, its immunogenicities using different nanoparticles adjuvants remain poorly understood.

Purpose: The study aimed to select an efficient nanoparticle antigen delivery system, which could enhance the immune responses of HCA59 in mice.

Methods: Here, the immune responses induced by the recombinant protein of HCA59 (rHCA59) with poly-D,L-lactide-co-glycolide (PLGA) nanoparticles, Chitosan nanoparticles, mixture of PLGA and Chitosan nanoparticles (rHCA59-Chitosan-PLGA), and Freund's complete adjuvant were observed, respectively, in mice. Cytokine and antibody levels induced by different groups were detected by ELISA assay. The effects of lymphocyte proliferations on different groups were examined using CCK-8 kit. Phenotypes of T cells and dendritic cells were analyzed by flow cytometry.

Results: On day 14 post vaccination, levels of IgM, IgG1, IgG2a, IFN-γ, IL-4, and IL-17 were significantly increased in the groups immunized with rHCA59 encapsulated with nanoparticles. After mice were vaccinated with rHCA59 loaded with Chitosan/PLGA nanoparticles, lymphocytes proliferated significantly. Additionally, the percentages of CD4 T cells (CD3 CD4), CD8 T cells (CD3 CD8), and dendritic cells (CD11c CD83, CD11c CD86) were obviously up-regulated in the mice immunized with nanoparticles, especially in the rHCA59-Chitosan-PLGA antigen delivery system group.

Conclusion: The findings of this research demonstrated that rHCA59-Chitosan-PLGA antigen delivery system could induce higher immune responses in mice model and indicated that rHCA59 might be a good candidate molecule to develop nanovaccines against in future study.

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