Recombinant Protein of Small GTPase ADP-ribosylation Factor 1 (HcARF1) Modulate the Cell Mediated Immune Response

Overview

Journal Oncotarget

Specialty Oncology

Date 2018 Jan 20

PMID 29348819

Citations 10

Authors

Javaid Ali Gadahi

Muhammad Ehsan

Shuai Wang

Zhenchao Zhang

Ruofeng Yan

Xiaokai Song

Lixin Xu

Xiangrui Li

Affiliations

Soon will be listed here.

Abstract

ADP-ribosylation factors (ARFs) are members of the Ras-related small GTPase family involved in the vesicular trafficking regulation. Immunomodulatory effects of these proteinson host cell arenot being addressed yet. small GTPase ADP-ribosylation 1 gene (HcARF1) was cloned and recombinant protein of HcARF1 (rHcARF1) was successfully expressed in . Binding activity of rHcARF1 to goat PBMCs was confirmed by immunofluorescence assay (IFA) and its immunomudulatory effects on cytokine secretion, cell proliferation, cell migration and nitric oxide production (NO) were observed by co-incubation of rHcARF1. IFA results revealed that rHcARF1 could bind to the PBMCs. The interaction of rHcARF1 modulated the cytokine production, the production of IL-4, IL-10 and IL-17 was increased in a dose dependent manner, however, the IFN-γ production was significantly decreased. Cell migration and NO production were significantly increased by rHcARF1, whereas, rHcARF1 treatment significantly suppressed the proliferation of the PBMC in a dose dependent manner. Our findings showed that the rHcARF1 play important roles on the goat PBMCs.

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References

Price H, Peltan A, Stark M, Smith D . The small GTPase ARL2 is required for cytokinesis in Trypanosoma brucei. Mol Biochem Parasitol. 2010; 173(2):123-31. PMC: 2913242. DOI: 10.1016/j.molbiopara.2010.05.016. View

Miljkovic D, Cvetkovic I, Vuckovic O, Stosic-Grujicic S, Mostarica Stojkovic M, Trajkovic V . The role of interleukin-17 in inducible nitric oxide synthase-mediated nitric oxide production in endothelial cells. Cell Mol Life Sci. 2003; 60(3):518-25. PMC: 11138561. DOI: 10.1007/s000180300043. View

Nahrevanian H . Involvement of nitric oxide and its up/down stream molecules in the immunity against parasitic infections. Braz J Infect Dis. 2010; 13(6):440-8. DOI: 10.1590/s1413-86702009000600010. View

Schlienger S, Ramirez R, Claing A . ARF1 regulates adhesion of MDA-MB-231 invasive breast cancer cells through formation of focal adhesions. Cell Signal. 2014; 27(3):403-15. DOI: 10.1016/j.cellsig.2014.11.032. View

Bruntz R, Lindsley C, Brown H . Phospholipase D signaling pathways and phosphatidic acid as therapeutic targets in cancer. Pharmacol Rev. 2014; 66(4):1033-79. PMC: 4180337. DOI: 10.1124/pr.114.009217. View

Cook W, Smith C, Senkovich O, Holder A, Chattopadhyay D . Structure of Plasmodium falciparum ADP-ribosylation factor 1. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010; 66(Pt 11):1426-31. PMC: 3001640. DOI: 10.1107/S1744309110036997. View

Gadahi J, Wang S, Bo G, Ehsan M, Yan R, Song X . Proteomic Analysis of the Excretory and Secretory Proteins of Haemonchus contortus (HcESP) Binding to Goat PBMCs In Vivo Revealed Stage-Specific Binding Profiles. PLoS One. 2016; 11(7):e0159796. PMC: 4965049. DOI: 10.1371/journal.pone.0159796. View

STAFFORD W, Stockley R, Ludbrook S, Holder A . Isolation, expression and characterization of the gene for an ADP-ribosylation factor from the human malaria parasite, Plasmodium falciparum. Eur J Biochem. 1996; 242(1):104-13. DOI: 10.1111/j.1432-1033.1996.0104r.x. View

Sahin A, Espiau B, Tetaud E, Cuvillier A, Lartigue L, Ambit A . The leishmania ARL-1 and Golgi traffic. PLoS One. 2008; 3(2):e1620. PMC: 2237903. DOI: 10.1371/journal.pone.0001620. View

10.

Volpicelli-Daley L, Li Y, Zhang C, Kahn R . Isoform-selective effects of the depletion of ADP-ribosylation factors 1-5 on membrane traffic. Mol Biol Cell. 2005; 16(10):4495-508. PMC: 1237059. DOI: 10.1091/mbc.e04-12-1042. View

11.

Kaminsky R, Ducray P, Jung M, Clover R, Rufener L, Bouvier J . A new class of anthelmintics effective against drug-resistant nematodes. Nature. 2008; 452(7184):176-80. DOI: 10.1038/nature06722. View

12.

Larkin B, Smith P, Ponichtera H, Shainheit M, Rutitzky L, Stadecker M . Induction and regulation of pathogenic Th17 cell responses in schistosomiasis. Semin Immunopathol. 2012; 34(6):873-88. PMC: 3690599. DOI: 10.1007/s00281-012-0341-9. View

13.

McSorley H, Maizels R . Helminth infections and host immune regulation. Clin Microbiol Rev. 2012; 25(4):585-608. PMC: 3485755. DOI: 10.1128/CMR.05040-11. View

14.

Jones D, Morris J, Morgan C, Kondo H, Irvine R, Cockcroft S . Type I phosphatidylinositol 4-phosphate 5-kinase directly interacts with ADP-ribosylation factor 1 and is responsible for phosphatidylinositol 4,5-bisphosphate synthesis in the golgi compartment. J Biol Chem. 2000; 275(18):13962-6. DOI: 10.1074/jbc.c901019199. View

15.

Adalid-Peralta L, Fragoso G, Fleury A, Sciutto E . Mechanisms underlying the induction of regulatory T cells and its relevance in the adaptive immune response in parasitic infections. Int J Biol Sci. 2011; 7(9):1412-26. PMC: 3221948. DOI: 10.7150/ijbs.7.1412. View

16.

Boulakirba S, Macia E, Partisani M, Lacas-Gervais S, Brau F, Luton F . Arf6 exchange factor EFA6 and endophilin directly interact at the plasma membrane to control clathrin-mediated endocytosis. Proc Natl Acad Sci U S A. 2014; 111(26):9473-8. PMC: 4084483. DOI: 10.1073/pnas.1401186111. View

17.

Li Y, Kelly W, Logsdon Jr J, Schurko A, Harfe B, Hill-Harfe K . Functional genomic analysis of the ADP-ribosylation factor family of GTPases: phylogeny among diverse eukaryotes and function in C. elegans. FASEB J. 2004; 18(15):1834-50. DOI: 10.1096/fj.04-2273com. View

18.

Price H, Stark M, Smith D . Trypanosoma brucei ARF1 plays a central role in endocytosis and golgi-lysosome trafficking. Mol Biol Cell. 2006; 18(3):864-73. PMC: 1805098. DOI: 10.1091/mbc.e06-08-0736. View

19.

Wu J, Kuo C . Pivotal role of ADP-ribosylation factor 6 in Toll-like receptor 9-mediated immune signaling. J Biol Chem. 2011; 287(6):4323-34. PMC: 3281705. DOI: 10.1074/jbc.M111.295113. View

20.

Russell J, Powers J, Rounbehler R, Rogers P, Conti C, Johnson D . ARF differentially modulates apoptosis induced by E2F1 and Myc. Mol Cell Biol. 2002; 22(5):1360-8. PMC: 134697. DOI: 10.1128/MCB.22.5.1360-1368.2002. View