IncRNA MAPKAPK5-AS1 Promotes Proliferation and Migration of Thyroid Cancer Cell Lines by Targeting MiR-519e-5p/YWHAH

Overview

Journal Eur J Histochem

Specialty Biochemistry

Date 2020 Dec 4

PMID 33272009

Citations 15

Authors

Yan Zhou

Shanshan Liu

Yan Luo

Meiying Zhang

Xueling Jiang

Yan Xiong

Affiliations

Soon will be listed here.

Abstract

Thyroid cancer is a common malignant tumour of the endocrine system and ranks ninth in cancer incidence worldwide. An extensive body of evidence has demonstrated that lncRNAs play a critical role in the progression of thyroid cancer. The lncRNA MAPKAPK5-AS1 has been reported to be abnormally expressed and to play a role in the development of various human cancers. However, MAPKAPK5-AS1's potential role in thyroid cancer progression remains unknown. The objective of our study was to explore the role and mechanism of MAPKAPK5-AS1 in thyroid cancer cells and provide a potential target for its biological diagnosis and treatment. We transfected sh-MAPKAPK5-AS1 and sh-NC into BCPAP and TPC-1 cells for loss-of-function assays. Results of RT-qPCR analysis demonstrated that MAPKAPK5-AS1 was more highly expressed in thyroid cancer cells compared to normal cells. Functional assays demonstrated that interfering with the expression of MAPKAPK5-AS1 notably repressed proliferation and invasion and accelerated apoptosis of BCPAP and TPC-1 cells. Mechanistically, we found that miR-519e-5p was negatively regulated by MAPKAPK5-AS1 and that tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta (YWHAH) was a target of miR-519e-5p. Additionally, rescue assays demonstrated that downregulation of MAPKAPK5-AS1 expression inhibited cell proliferation, migration, and invasion and promoted apoptosis by sponging miR-519e-5p, thereby increasing YWHAH expression. Ultimately, our study revealed that MAPKAPK5-AS1 promotes proliferation and migration of thyroid cancer cells by targeting the miR-519e-5p/YWHAH axis, which provides novel insight into the development and progression of thyroid cancer.

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References

Chen W, Zhu H, Yin L, Wang T, Wu J, Xu J . lncRNA-PVT1 Facilitates Invasion Through Upregulation of MMP9 in Nonsmall Cell Lung Cancer Cell. DNA Cell Biol. 2017; 36(9):787-793. DOI: 10.1089/dna.2017.3725. View

Gu Z, Hou Z, Zheng L, Wang X, Wu L, Zhang C . LncRNA DICER1-AS1 promotes the proliferation, invasion and autophagy of osteosarcoma cells via miR-30b/ATG5. Biomed Pharmacother. 2018; 104:110-118. DOI: 10.1016/j.biopha.2018.04.193. View

Yu G, Zhang T, Jing Y, Bao Q, Tang Q, Zhang Y . miR-519 suppresses nasopharyngeal carcinoma cell proliferation by targeting oncogene URG4/URGCP. Life Sci. 2017; 175:47-51. DOI: 10.1016/j.lfs.2017.03.010. View

Cramer J, Fu P, Harth K, Margevicius S, Wilhelm S . Analysis of the rising incidence of thyroid cancer using the Surveillance, Epidemiology and End Results national cancer data registry. Surgery. 2010; 148(6):1147-52. DOI: 10.1016/j.surg.2010.10.016. View

Aprelikova O, Yu X, Palla J, Wei B, John S, Yi M . The role of miR-31 and its target gene SATB2 in cancer-associated fibroblasts. Cell Cycle. 2010; 9(21):4387-98. PMC: 3055190. DOI: 10.4161/cc.9.21.13674. View

Huang K, Geng J, Wang J . Long non-coding RNA RP11-552M11.4 promotes cells proliferation, migration and invasion by targeting BRCA2 in ovarian cancer. Cancer Sci. 2018; 109(5):1428-1446. PMC: 5980309. DOI: 10.1111/cas.13552. View

Haonon O, Rucksaken R, Pinlaor P, Pairojkul C, Chamgramol Y, Intuyod K . Upregulation of 14-3-3 eta in chronic liver fluke infection is a potential diagnostic marker of cholangiocarcinoma. Proteomics Clin Appl. 2015; 10(3):248-56. DOI: 10.1002/prca.201500019. View

Yang T, Chen W, Shi P, Liu M, Jiang T, Song H . Long noncoding RNA MAPKAPK5-AS1 promotes colorectal cancer progression by cis-regulating the nearby gene MK5 and acting as a let-7f-1-3p sponge. J Exp Clin Cancer Res. 2020; 39(1):139. PMC: 7370515. DOI: 10.1186/s13046-020-01633-8. View

Zhang M, Wu W, Wang Z, Wang X . lncRNA NEAT1 is closely related with progression of breast cancer via promoting proliferation and EMT. Eur Rev Med Pharmacol Sci. 2017; 21(5):1020-1026. View

10.

Zhao X, Wang D, Ding Y, Zhou J, Liu G, Ji Z . lncRNA ZEB1-AS1 promotes migration and metastasis of bladder cancer cells by post-transcriptional activation of ZEB1. Int J Mol Med. 2019; 44(1):196-206. PMC: 6559313. DOI: 10.3892/ijmm.2019.4187. View

11.

Ren L, Li Y, Zhao Q, Fan L, Tan B, Zang A . miR-519 regulates the proliferation of breast cancer cells via targeting human antigen R. Oncol Lett. 2020; 19(2):1567-1576. PMC: 6956239. DOI: 10.3892/ol.2019.11230. View

12.

Liu Q, Guo X, Que S, Yang X, Fan H, Liu M . LncRNA RSU1P2 contributes to tumorigenesis by acting as a ceRNA against let-7a in cervical cancer cells. Oncotarget. 2016; 8(27):43768-43781. PMC: 5546439. DOI: 10.18632/oncotarget.10844. View

13.

Yuan Q, Liu Y, Fan Y, Liu Z, Wang X, Jia M . LncRNA HOTTIP promotes papillary thyroid carcinoma cell proliferation, invasion and migration by regulating miR-637. Int J Biochem Cell Biol. 2018; 98:1-9. DOI: 10.1016/j.biocel.2018.02.013. View

14.

Li X, Wang S, Li Z, Long X, Guo Z, Zhang G . The lncRNA NEAT1 facilitates cell growth and invasion via the miR-211/HMGA2 axis in breast cancer. Int J Biol Macromol. 2017; 105(Pt 1):346-353. DOI: 10.1016/j.ijbiomac.2017.07.053. View

15.

Li Y, Shao J, Zhang S, Xing G, Liu H . miR-519d-3p Inhibits Cell Proliferation and Invasion of Gastric Cancer by Downregulating B-Cell Lymphoma 6. Cytogenet Genome Res. 2018; 154(1):12-19. PMC: 5969071. DOI: 10.1159/000487372. View

16.

Zhou J, Zheng S, Liu J, Shi R, Yu H, Wei M . MiR-519d facilitates the progression and metastasis of cervical cancer through direct targeting Smad7. Cancer Cell Int. 2016; 16:21. PMC: 4802873. DOI: 10.1186/s12935-016-0298-1. View

17.

Jin Y, Feng S, Qiu S, Shao N, Zheng J . LncRNA MALAT1 promotes proliferation and metastasis in epithelial ovarian cancer via the PI3K-AKT pathway. Eur Rev Med Pharmacol Sci. 2017; 21(14):3176-3184. View

18.

Zhang H, Wei Q, Wang S, Zhang H, Zhang X, Geng Q . LncRNA HOTAIR alleviates rheumatoid arthritis by targeting miR-138 and inactivating NF-κB pathway. Int Immunopharmacol. 2017; 50:283-290. DOI: 10.1016/j.intimp.2017.06.021. View

19.

Liu D, Li Y, Luo G, Xiao X, Tao D, Wu X . LncRNA SPRY4-IT1 sponges miR-101-3p to promote proliferation and metastasis of bladder cancer cells through up-regulating EZH2. Cancer Lett. 2016; 388:281-291. DOI: 10.1016/j.canlet.2016.12.005. View

20.

Jiang H, Liang M, Jiang Y, Zhang T, Mo K, Su S . The lncRNA TDRG1 promotes cell proliferation, migration and invasion by targeting miR-326 to regulate MAPK1 expression in cervical cancer. Cancer Cell Int. 2019; 19:152. PMC: 6544966. DOI: 10.1186/s12935-019-0872-4. View