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MiR-519d Facilitates the Progression and Metastasis of Cervical Cancer Through Direct Targeting Smad7

Overview
Journal Cancer Cell Int
Publisher Biomed Central
Date 2016 Mar 24
PMID 27006642
Citations 26
Authors
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Abstract

Background: MicroRNAs (miRNAs) play pivotal roles in the development of various cancer types, including cervical cancer.

Methods And Results: In this study, we showed that miR-519d, a miRNA within the chromosome 19 miRNA cluster, was significantly upregulated in cervical cancer tissues, compared with non-tumorous cervical samples. Suppression of miR-519d markedly attenuated the migration and invasion of HeLa and SiHa cervical cancer cells. Additionally, miR-519d inhibited the apoptosis of cervical cancer cells, and the proliferation of cervical cancer cells was also affected following transfection of miR-519d inhibitor. Moreover, we identified Smad7 to be a novel target of miR-519d in cervical cancer cells. MiR-519d matched the 3'-UTR of Smad7 mRNA. Transfection with miR-519d mimics led to apparent downregulation of Smad7 both at the mRNA and protein levels. Luciferase reporter analysis revealed that miR-519d reduced the luciferase activity of Smad7 mRNA 3'-UTR through matching site-dependent manner. And more notably, suppression of Smad7 remarkably restored the migration and invasion of miR-519d-depleted cervical cancer cells.

Conclusion: Taken together, these findings implicated that miR-519d promoted the progression and metastasis of cervical cancer through targeting Smad7.

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References
1.
Diaz-Padilla I, Monk B, MacKay H, Oaknin A . Treatment of metastatic cervical cancer: future directions involving targeted agents. Crit Rev Oncol Hematol. 2012; 85(3):303-14. DOI: 10.1016/j.critrevonc.2012.07.006. View

2.
Lehmann U, Streichert T, Otto B, Albat C, Hasemeier B, Christgen H . Identification of differentially expressed microRNAs in human male breast cancer. BMC Cancer. 2010; 10:109. PMC: 2850898. DOI: 10.1186/1471-2407-10-109. View

3.
Inui M, Martello G, Piccolo S . MicroRNA control of signal transduction. Nat Rev Mol Cell Biol. 2010; 11(4):252-63. DOI: 10.1038/nrm2868. View

4.
Pang Y, Mao H, Shen L, Zhao Z, Liu R, Liu P . MiR-519d represses ovarian cancer cell proliferation and enhances cisplatin-mediated cytotoxicity in vitro by targeting XIAP. Onco Targets Ther. 2014; 7:587-97. PMC: 4003267. DOI: 10.2147/OTT.S60289. View

5.
Wang X, Tang S, Le S, Lu R, Rader J, Meyers C . Aberrant expression of oncogenic and tumor-suppressive microRNAs in cervical cancer is required for cancer cell growth. PLoS One. 2008; 3(7):e2557. PMC: 2438475. DOI: 10.1371/journal.pone.0002557. View