IMPLICATIONS OF YWHAH GENE EXPRESSION IN THE EARLY DETECTION OF SEPSIS

Overview

Journal Shock

Date 2024 Jun 21

PMID 38904460

Authors

Hua Yao

Yue Zhou

Zhengguang Geng

Fei Gao

De Su

Yan Kang

Bao Fu

Affiliations

Soon will be listed here.

Abstract

Sepsis, a complex and multifaceted condition, is a common occurrence with serious implications for critically ill patients in the intensive care unit (ICU). The YWHAH gene encodes the 14-3-3n protein, a member of the 14-3-3 protein family. While existing research primarily focuses on the role of 14-3-3n in conditions such as schizophrenia and various cancers, our study revealed that the expression of the YWHAH gene remained relatively stable in both infected individuals and healthy controls. Through Venn plot analysis following weighted gene correlation network analysis, we observed a potential association between elevated YWHAH expression and the transition from infection to sepsis. In a comprehensive analysis of public single-cell transcriptome databases, the expression of YWHAH was found to be distinctive in cases of sepsis and infection. These findings were corroborated through an in vitro analysis utilizing real-time polymerase chain reaction. This study represents the initial identification of variations in YWHAH gene expression between patients with infection and sepsis, potentially offering insights for the development of early detection and treatment strategies for sepsis.

References

Li X, Xu Z, Pang X, Huang Y, Yang B, Yang Y . Interleukin-10/lymphocyte ratio predicts mortality in severe septic patients. PLoS One. 2017; 12(6):e0179050. PMC: 5476240. DOI: 10.1371/journal.pone.0179050. View

Song Z, Zhang J, Zhang X, Li D, Wang H, Xu X . Interleukin 4 Deficiency Reverses Development of Secondary Pseudomonas aeruginosa Pneumonia During Sepsis-Associated Immunosuppression. J Infect Dis. 2014; 211(10):1616-27. DOI: 10.1093/infdis/jiu668. View

Sivayoham N, Rhodes A, Cecconi M . The MISSED score, a new scoring system to predict Mortality In Severe Sepsis in the Emergency Department: a derivation and validation study. Eur J Emerg Med. 2013; 21(1):30-6. DOI: 10.1097/MEJ.0b013e328364a8d4. View

Yu G, Wang L, Han Y, He Q . clusterProfiler: an R package for comparing biological themes among gene clusters. OMICS. 2012; 16(5):284-7. PMC: 3339379. DOI: 10.1089/omi.2011.0118. View

Usul E, Korkut S, Kayipmaz A, Halici A, Kavalci C . The role of the quick sequential organ failure assessment score (qSOFA) and modified early warning score (MEWS) in the pre-hospitalization prediction of sepsis prognosis. Am J Emerg Med. 2020; 41:158-162. DOI: 10.1016/j.ajem.2020.09.049. View

Fleischmann C, Thomas-Rueddel D, Hartmann M, Hartog C, Welte T, Heublein S . Hospital Incidence and Mortality Rates of Sepsis. Dtsch Arztebl Int. 2016; 113(10):159-66. PMC: 4814768. DOI: 10.3238/arztebl.2016.0159. View

Puskarich M, Trzeciak S, Shapiro N, Arnold R, Heffner A, Kline J . Prognostic value and agreement of achieving lactate clearance or central venous oxygen saturation goals during early sepsis resuscitation. Acad Emerg Med. 2012; 19(3):252-8. PMC: 3313083. DOI: 10.1111/j.1553-2712.2012.01292.x. View

Szklarczyk D, Gable A, Lyon D, Junge A, Wyder S, Huerta-Cepas J . STRING v11: protein-protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets. Nucleic Acids Res. 2018; 47(D1):D607-D613. PMC: 6323986. DOI: 10.1093/nar/gky1131. View

Ali A, Na M, Svensson M, Magnusson M, Welin A, Schwarze J . IL-1 Receptor Antagonist Treatment Aggravates Staphylococcal Septic Arthritis and Sepsis in Mice. PLoS One. 2015; 10(7):e0131645. PMC: 4489902. DOI: 10.1371/journal.pone.0131645. View

10.

Rubulotta F, Marshall J, Ramsay G, Nelson D, Levy M, Williams M . Predisposition, insult/infection, response, and organ dysfunction: A new model for staging severe sepsis. Crit Care Med. 2009; 37(4):1329-35. DOI: 10.1097/CCM.0b013e31819d5db1. View

11.

Chaudhry H, Zhou J, Zhong Y, Ali M, McGuire F, Nagarkatti P . Role of cytokines as a double-edged sword in sepsis. In Vivo. 2013; 27(6):669-84. PMC: 4378830. View

12.

Gouel-Cheron A, Allaouchiche B, Guignant C, Davin F, Floccard B, Monneret G . Early interleukin-6 and slope of monocyte human leukocyte antigen-DR: a powerful association to predict the development of sepsis after major trauma. PLoS One. 2012; 7(3):e33095. PMC: 3303782. DOI: 10.1371/journal.pone.0033095. View

13.

Kim J, Kang J, Kang Y, Woo J, Kim Y, Huh J . Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nat Commun. 2020; 11(1):5436. PMC: 7595112. DOI: 10.1038/s41467-020-19263-1. View

14.

Vincent J . The Clinical Challenge of Sepsis Identification and Monitoring. PLoS Med. 2016; 13(5):e1002022. PMC: 4871479. DOI: 10.1371/journal.pmed.1002022. View

15.

Quinten V, van Meurs M, Wolffensperger A, Ter Maaten J, Ligtenberg J . Sepsis patients in the emergency department: stratification using the Clinical Impression Score, Predisposition, Infection, Response and Organ dysfunction score or quick Sequential Organ Failure Assessment score?. Eur J Emerg Med. 2017; 25(5):328-334. PMC: 6133213. DOI: 10.1097/MEJ.0000000000000460. View

16.

Hubbard W, Choudhry M, Schwacha M, Kerby J, Rue 3rd L, Bland K . Cecal ligation and puncture. Shock. 2005; 24 Suppl 1:52-7. DOI: 10.1097/01.shk.0000191414.94461.7e. View

17.

Mishra S, Choudhury S . Experimental Protocol for Cecal Ligation and Puncture Model of Polymicrobial Sepsis and Assessment of Vascular Functions in Mice. Methods Mol Biol. 2018; 1717:161-187. DOI: 10.1007/978-1-4939-7526-6_14. View

18.

Wu Y, Zhang Y, Wang F, Ni Q, Li M . MiR-660-5p promotes the progression of hepatocellular carcinoma by interaction with YWHAH via PI3K/Akt signaling pathway. Biochem Biophys Res Commun. 2020; 531(4):480-489. DOI: 10.1016/j.bbrc.2020.07.034. View

19.

Morrison D . The 14-3-3 proteins: integrators of diverse signaling cues that impact cell fate and cancer development. Trends Cell Biol. 2008; 19(1):16-23. PMC: 3073487. DOI: 10.1016/j.tcb.2008.10.003. View

20.

Mera S, tatulescu D, Cismaru C, Bondor C, Slavcovici A, Zanc V . Multiplex cytokine profiling in patients with sepsis. APMIS. 2011; 119(2):155-63. DOI: 10.1111/j.1600-0463.2010.02705.x. View