Liver-expressed and Limit Hepatitis C Virus Cross-species Transmission to Mice

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2020 Nov 5

PMID 33148654

Citations 13

Authors

Richard J P Brown

Birthe Tegtmeyer

Julie Sheldon

Tanvi Khera

Anggakusuma

Daniel Todt

Gabrielle Vieyres

Romy Weller

Sebastian Joecks

Yudi Zhang

Svenja Sake

Dorothea Bankwitz

Kathrin Welsch

Corinne Ginkel

Michael Engelmann

Gisa Gerold

Eike Steinmann

Qinggong Yuan

Michael Ott

Florian W R Vondran

Thomas Krey

Luisa J Stroh

Csaba Miskey

Zoltan Ivics

Vanessa Herder

Wolfgang Baumgartner

Chris Lauber

Michael Seifert

Alexander W Tarr

C Patrick McClure

Glenn Randall

Yasmine Baktash

Alexander Ploss

Viet Loan Dao Thi

Eleftherios Michailidis

Mohsan Saeed

Lieven Verhoye

Philip Meuleman

Natascha Goedecke

Dagmar Wirth

Charles M Rice

Thomas Pietschmann

Affiliations

Soon will be listed here.

Abstract

Hepatitis C virus (HCV) has no animal reservoir, infecting only humans. To investigate species barrier determinants limiting infection of rodents, murine liver complementary DNA library screening was performed, identifying transmembrane proteins Cd302 and Cr1l as potent restrictors of HCV propagation. Combined ectopic expression in human hepatoma cells impeded HCV uptake and cooperatively mediated transcriptional dysregulation of a noncanonical program of immunity genes. Murine hepatocyte expression of both factors was constitutive and not interferon inducible, while differences in liver expression and the ability to restrict HCV were observed between the murine orthologs and their human counterparts. Genetic ablation of endogenous expression in human HCV entry factor transgenic mice increased hepatocyte permissiveness for an adapted HCV strain and dysregulated expression of metabolic process and host defense genes. These findings highlight human-mouse differences in liver-intrinsic antiviral immunity and facilitate the development of next-generation murine models for preclinical testing of HCV vaccine candidates.

Citing Articles

TMPRSS2-mediated SARS-CoV-2 uptake boosts innate immune activation, enhances cytopathology, and drives convergent virus evolution.

Qu B, Miskey C, Gomer A, Kleinert R, Ibanez S, Eberle R Proc Natl Acad Sci U S A. 2024; 121(23):e2407437121.

PMID: 38814864 PMC: 11161796. DOI: 10.1073/pnas.2407437121.

Unraveling the dynamics of hepatitis C virus adaptive mutations and their impact on antiviral responses in primary human hepatocytes.

Frericks N, Brown R, Reinecke B, Herrmann M, Bruggemann Y, Todt D J Virol. 2024; 98(3):e0192123.

PMID: 38319104 PMC: 10949430. DOI: 10.1128/jvi.01921-23.

Mouse Liver-Expressed Shiftless Is an Evolutionarily Conserved Antiviral Effector Restricting Human and Murine Hepaciviruses.

Zhang Y, Kinast V, Sheldon J, Frericks N, Todt D, Zimmer M Microbiol Spectr. 2023; 11(4):e0128423.

PMID: 37341610 PMC: 10433982. DOI: 10.1128/spectrum.01284-23.

Vaccine-associated enhanced respiratory pathology in COVID-19 hamsters after T2-biased immunization.

Ebenig A, Muraleedharan S, Kazmierski J, Todt D, Auste A, Anzaghe M Cell Rep. 2022; 40(7):111214.

PMID: 35952673 PMC: 9346010. DOI: 10.1016/j.celrep.2022.111214.

The Human Liver-Expressed Lectin CD302 Restricts Hepatitis C Virus Infection.

Reinecke B, Frericks N, Lauber C, Dinkelborg K, Matthaei A, Vondran F J Virol. 2022; 96(7):e0199521.

PMID: 35297672 PMC: 9006913. DOI: 10.1128/jvi.01995-21.

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