A Promising IPS-based Single-cell Cloning Strategy Revealing Signatures of Somatic Mutations in Heterogeneous Normal Cells

Overview

Journal Comput Struct Biotechnol J

Specialty Biotechnology

Date 2020 Sep 30

PMID 32994891

Authors

Xuexia Miao

Yueying Li

Caihong Zheng

Lifei Wang

Chen Jin

Lei Chen

Shuangli Mi

Weiwei Zhai

Qian-Fei Wang

Jun Cai

Affiliations

Soon will be listed here.

Abstract

Single-cell genomics has advanced rapidly as trace-DNA amplification technologies evolved. However, current technologies are subject to a variety of pitfalls such as contamination, uneven genomic coverage, and amplification errors. Even for the "golden" strategy of single stem cell-derived clonal formation, high-fidelity amplification is applicable merely to single stem cells. It's still challenging to accurately define somatic mutations of a single cell in various cell types. Herein, we provided evidence, for the first time, to prove that induced pluripotent stem cells (iPS cells or iPSC), being a single somatic cell-derived clone, are recording almost identical (>90%) mutational profile of the initial cell progenitor. This finding demonstrates iPS technique, applicable to any cell type, can be utilized as a cell cloning strategy favorable for single-cell genomic amplification. This novel strategy is not limited by cell-type constraints or amplification artifacts, and thus enables our detailed investigation on the characteristics of somatic mutations in heterogeneous normal cells.

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