Methods for Making Induced Pluripotent Stem Cells: Reprogramming à La Carte

Overview

Journal Nat Rev Genet

Specialty Genetics

Date 2011 Feb 23

PMID 21339765

Citations 220

Authors

Federico Gonzalez

Stephanie Boue

Juan Carlos Izpisua Belmonte

Affiliations

Soon will be listed here.

Abstract

Pluripotent stem-cell lines can be obtained through the reprogramming of somatic cells from different tissues and species by ectopic expression of defined factors. In theory, these cells--known as induced pluripotent stem cells (iPSCs)--are suitable for various purposes, including disease modelling, autologous cell therapy, drug or toxicity screening and basic research. Recent methodological improvements are increasing the ease and efficiency of reprogramming, and reducing the genomic modifications required to complete the process. However, depending on the downstream applications, certain technologies have advantages over others. Here, we provide a comprehensive overview of the existing reprogramming approaches with the aim of providing readers with a better understanding of the reprogramming process and a basis for selecting the most suitable method for basic or clinical applications.

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References

Hotta A, Ellis J . Retroviral vector silencing during iPS cell induction: an epigenetic beacon that signals distinct pluripotent states. J Cell Biochem. 2008; 105(4):940-8. DOI: 10.1002/jcb.21912. View

Takahashi K, Okita K, Nakagawa M, Yamanaka S . Induction of pluripotent stem cells from fibroblast cultures. Nat Protoc. 2007; 2(12):3081-9. DOI: 10.1038/nprot.2007.418. View

Li W, Zhou H, Abujarour R, Zhu S, Joo J, Lin T . Generation of human-induced pluripotent stem cells in the absence of exogenous Sox2. Stem Cells. 2009; 27(12):2992-3000. PMC: 3780784. DOI: 10.1002/stem.240. View

Ellis J, Bruneau B, Keller G, Lemischka I, Nagy A, Rossant J . Alternative induced pluripotent stem cell characterization criteria for in vitro applications. Cell Stem Cell. 2009; 4(3):198-9. DOI: 10.1016/j.stem.2009.02.010. View

Silva J, Barrandon O, Nichols J, Kawaguchi J, Theunissen T, Smith A . Promotion of reprogramming to ground state pluripotency by signal inhibition. PLoS Biol. 2008; 6(10):e253. PMC: 2570424. DOI: 10.1371/journal.pbio.0060253. View

Jahner D, Stuhlmann H, Stewart C, Harbers K, Lohler J, Simon I . De novo methylation and expression of retroviral genomes during mouse embryogenesis. Nature. 1982; 298(5875):623-8. DOI: 10.1038/298623a0. View

Woltjen K, Michael I, Mohseni P, Desai R, Mileikovsky M, Hamalainen R . piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature. 2009; 458(7239):766-70. PMC: 3758996. DOI: 10.1038/nature07863. View

Okita K, Ichisaka T, Yamanaka S . Generation of germline-competent induced pluripotent stem cells. Nature. 2007; 448(7151):313-7. DOI: 10.1038/nature05934. View

Wilson M, Coates C, George Jr A . PiggyBac transposon-mediated gene transfer in human cells. Mol Ther. 2006; 15(1):139-45. DOI: 10.1038/sj.mt.6300028. View

10.

Zhao Y, Yin X, Qin H, Zhu F, Liu H, Yang W . Two supporting factors greatly improve the efficiency of human iPSC generation. Cell Stem Cell. 2008; 3(5):475-9. DOI: 10.1016/j.stem.2008.10.002. View

11.

Wernig M, Meissner A, Cassady J, Jaenisch R . c-Myc is dispensable for direct reprogramming of mouse fibroblasts. Cell Stem Cell. 2008; 2(1):10-2. DOI: 10.1016/j.stem.2007.12.001. View

12.

Chen Z, He C, Kay M . Improved production and purification of minicircle DNA vector free of plasmid bacterial sequences and capable of persistent transgene expression in vivo. Hum Gene Ther. 2005; 16(1):126-31. DOI: 10.1089/hum.2005.16.126. View

13.

Cary L, Goebel M, Corsaro B, Wang H, Rosen E, Fraser M . Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses. Virology. 1989; 172(1):156-69. DOI: 10.1016/0042-6822(89)90117-7. View

14.

Carey B, Markoulaki S, Hanna J, Saha K, Gao Q, Mitalipova M . Reprogramming of murine and human somatic cells using a single polycistronic vector. Proc Natl Acad Sci U S A. 2008; 106(1):157-62. PMC: 2629226. DOI: 10.1073/pnas.0811426106. View

15.

Kim J, Sebastiano V, Wu G, Arauzo-Bravo M, Sasse P, Gentile L . Oct4-induced pluripotency in adult neural stem cells. Cell. 2009; 136(3):411-9. DOI: 10.1016/j.cell.2009.01.023. View

16.

Fraser M, Cary L, Boonvisudhi K, Wang H . Assay for movement of Lepidopteran transposon IFP2 in insect cells using a baculovirus genome as a target DNA. Virology. 1995; 211(2):397-407. DOI: 10.1006/viro.1995.1422. View

17.

Feng B, Ng J, Heng J, Ng H . Molecules that promote or enhance reprogramming of somatic cells to induced pluripotent stem cells. Cell Stem Cell. 2009; 4(4):301-12. DOI: 10.1016/j.stem.2009.03.005. View

18.

Shi Y, Desponts C, Do J, Hahm H, Scholer H, Ding S . Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell. 2008; 3(5):568-74. DOI: 10.1016/j.stem.2008.10.004. View

19.

Park I, Zhao R, West J, Yabuuchi A, Huo H, Ince T . Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 2007; 451(7175):141-6. DOI: 10.1038/nature06534. View

20.

Michiue H, Tomizawa K, Wei F, Matsushita M, Lu Y, Ichikawa T . The NH2 terminus of influenza virus hemagglutinin-2 subunit peptides enhances the antitumor potency of polyarginine-mediated p53 protein transduction. J Biol Chem. 2004; 280(9):8285-9. DOI: 10.1074/jbc.M412430200. View