Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants

Overview

Journal ACS Appl Bio Mater

Specialties Biomedical Engineering
Biotechnology

Date 2020 Sep 28

PMID 32984779

Citations 10

Authors

Kathryn A Murray

Ruben M F Tomas

Matthew I Gibson

Affiliations

Soon will be listed here.

Abstract

Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.

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