The Ras-related Ral Gene Maps to Chromosome 7p15-22

Overview

Journal Hum Genet

Specialty Genetics

Date 1988 Jun 1

PMID 3292391

Citations 6

Authors

M F Rousseau-Merck

A Bernheim

P Chardin

R Miglierina

A Tavitian

R Berger

Affiliations

Soon will be listed here.

Abstract

Human cDNAs coding for the new protein ral that shares 50% homology with the ras proteins have been recently isolated. A 600-bp fragment carrying mainly the coding region was used to localize the ral gene by hybridization with sorted chromosomes and in situ hybridization. Direct molecular hybridization on sorted chromosomes using a single laser illumination allowed the assignment of the ral gene to a region of the flow karyotype containing chromosomes 7, 8 and X. With dual laser analysis ral was assigned to the fraction containing chromosome 7. In the 331 human metaphases hybridized with the 3H-labelled insert, the silver grain distribution showed a unique major signal on chromosome 7p15-22.

Citing Articles

Quantitative proteomic analysis of the frontal cortex in Alzheimer's disease.

Sathe G, Albert M, Darrow J, Saito A, Troncoso J, Pandey A J Neurochem. 2020; 156(6):988-1002.

PMID: 32614981 PMC: 7775912. DOI: 10.1111/jnc.15116.

Emerging RAS superfamily conditions involving GTPase function.

Shieh J PLoS Genet. 2019; 15(2):e1007870.

PMID: 30763311 PMC: 6375560. DOI: 10.1371/journal.pgen.1007870.

Chromosome assignment of four RAS-related RAB genes.

Rousseau-Merck M, Zahraoui A, Touchot N, Tavitian A, Berger R Hum Genet. 1991; 86(4):350-4.

PMID: 1999336 DOI: 10.1007/BF00201831.

Chromosomal localization of the human D3 dopamine receptor gene.

Le Coniat M, Sokoloff P, Hillion J, Martres M, Giros B, Pilon C Hum Genet. 1991; 87(5):618-20.

PMID: 1916765 DOI: 10.1007/BF00209024.

Mouse chromosome 13.

Justice M, Stephenson D Mamm Genome. 1991; 1 Spec No:S205-20.

PMID: 1799801 DOI: 10.1007/BF00656494.

References

de Taisne C, Gegonne A, Stehelin D, Bernheim A, Berger R . Chromosomal localization of the human proto-oncogene c-ets. Nature. 1984; 310(5978):581-3. DOI: 10.1038/310581a0. View

de Martinville B, Francke U . The c-Ha-ras1, insulin and beta-globin loci map outside the deletion associated with aniridia-Wilms' tumour. Nature. 1983; 305(5935):641-3. DOI: 10.1038/305641a0. View

Sillar R, Young B . A new method for the preparation of metaphase chromosomes for flow analysis. J Histochem Cytochem. 1981; 29(1):74-8. DOI: 10.1177/29.1.6162882. View

Lamm L, Olaisen B . Report of the Committee on the Genetic Constitution of Chromosomes 5 and 6. Cytogenet Cell Genet. 1985; 40(1-4):128-55. DOI: 10.1159/000132172. View

Cooper C, Park M, Blair D, Tainsky M, Huebner K, Croce C . Molecular cloning of a new transforming gene from a chemically transformed human cell line. Nature. 1984; 311(5981):29-33. DOI: 10.1038/311029a0. View

Bernheim A, Metezeau P, Guellaen G, Fellous M, Goldberg M, Berger R . Direct hybridization of sorted human chromosomes: localization of the Y chromosome on the flow karyotype. Proc Natl Acad Sci U S A. 1983; 80(24):7571-5. PMC: 534382. DOI: 10.1073/pnas.80.24.7571. View

Rabin M, A Ferguson-Smith , Hart C, Ruddle F . Cognate homeo-box loci mapped on homologous human and mouse chromosomes. Proc Natl Acad Sci U S A. 1986; 83(23):9104-8. PMC: 387083. DOI: 10.1073/pnas.83.23.9104. View

Huerre C, Despoisse S, Gilgenkrantz S, Lenoir G, Junien C . c-Ha-ras1 is not deleted in aniridia-Wilms' tumour association. Nature. 1983; 305(5935):638-41. DOI: 10.1038/305638a0. View

Berger R, Bloomfield C, Sutherland G . Report of the Committee on Chromosome Rearrangements in Neoplasia and on Fragile Sites. Cytogenet Cell Genet. 1985; 40(1-4):490-535. DOI: 10.1159/000132181. View

10.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

11.

Jhanwar S, Neel B, Hayward W, Chaganti R . Localization of c-ras oncogene family on human germ-line chromosomes. Proc Natl Acad Sci U S A. 1983; 80(15):4794-7. PMC: 384131. DOI: 10.1073/pnas.80.15.4794. View

12.

Harper M, Saunders G . Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981; 83(3):431-9. DOI: 10.1007/BF00327364. View

13.

Lowe D, Capon D, Delwart E, Sakaguchi A, Naylor S, Goeddel D . Structure of the human and murine R-ras genes, novel genes closely related to ras proto-oncogenes. Cell. 1987; 48(1):137-46. DOI: 10.1016/0092-8674(87)90364-3. View

14.

Caubet J, Bernheim A, Larsen C, Berger R . Human proto-oncogene c-mos maps to 8q11. EMBO J. 1985; 4(9):2245-8. PMC: 554492. DOI: 10.1002/j.1460-2075.1985.tb03921.x. View

15.

OBrien S, Nash W, Goodwin J, Lowy D, Chang E . Dispersion of the ras family of transforming genes to four different chromosomes in man. Nature. 1983; 302(5911):839-42. DOI: 10.1038/302839a0. View

16.

Grzeschik K, Kazazian H . Report of the Committee on the Genetic Constitution of Chromosomes 10, 11, and 12. Cytogenet Cell Genet. 1985; 40(1-4):179-205. DOI: 10.1159/000132174. View

17.

Barbacid M . ras genes. Annu Rev Biochem. 1987; 56:779-827. DOI: 10.1146/annurev.bi.56.070187.004023. View

18.

Povey S, Morton N, Sherman S . Report of the Committee on the Genetic Constitution of Chromosomes 1 and 2. Cytogenet Cell Genet. 1985; 40(1-4):67-106. DOI: 10.1159/000132170. View

19.

Madaule P, Axel R . A novel ras-related gene family. Cell. 1985; 41(1):31-40. DOI: 10.1016/0092-8674(85)90058-3. View

20.

Chardin P, Tavitian A . The ral gene: a new ras related gene isolated by the use of a synthetic probe. EMBO J. 1986; 5(9):2203-8. PMC: 1167101. DOI: 10.1002/j.1460-2075.1986.tb04485.x. View