Localization of Single Copy DNA Sequences of G-banded Human Chromosomes by in Situ Hybridization

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1981 Jan 1

PMID 7273954

Citations 304

Authors

M E Harper

G F Saunders

Affiliations

Soon will be listed here.

Abstract

Recombinant lambda bacteriophage clone H3 containing a human DNA segment of 14.9 kb present in one or two copies per haploid genome was isolated. In situ hybridization in human metaphase chromosomes of the 3H-labeled cloned DNA resulted in highly significant labeling (53% of cells) of band p36 of chromosome 1, such that 22% of all chromosomal grains were located on this region. Hybridization was dependent upon the presence of dextran sulfate in the hybridization mixture and was not affected by repetitive DNA competitor. These results demonstrate localization of a single copy sequence on human metaphase chromosomes.

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