LncRNA SNHG7 Sponges MiR-449a to Promote Pituitary Adenomas Progression

Overview

Journal Metab Brain Dis

Publisher Springer

Specialties Endocrinology
Neurology

Date 2020 Sep 4

PMID 32880813

Citations 9

Authors

Xiongfei Yue

Ce Dong

Zhanying Ye

Lin Zhu

Xiaoyang Zhang

Xiaoyan Wang

Feng Mo

Zheng Li

Baogen Pan

Affiliations

Soon will be listed here.

Abstract

This study aimed to characterize the expression status and potentially mechanistic involvement of SNHG7 in pituitary adenoma. Relative expression of SNHG7 and miR-449a was analyzed by real-time PCR. Cell viability was measured with Cell Counting Kit-8 (CCK-8). Cell apoptosis was determined by PI/Annexin V double staining followed by flow cytometry analysis. Cell invasion and migration were analyzed by wound healing and transwell assays, respectively. The regulatory action of miR-449a on SNHG7 was interrogated by luciferase reporter assay. We also investigated the pro-tumor activity of SNHG7 with the MMQ xenograft tumor mouse model. We identified the aberrant up-regulation of SNHG7 in pituitary adenoma both in vivo and in vitro, which associated with poor survival outcome. siRNA-mediated SNHG7-knockdown decreased cell viability, increased apoptosis and compromised migration and invasion. We further predicted and validated that SNHG7 negatively regulated miR-449a via sponging. Concurrent inhibition of miR-449a restored cell viability, apoptosis, migration and invasion influenced by SNHG7-deficiency. Most importantly, we demonstrated that SNHG7-silencing delayed xenograft tumor progression, which was accompanied with increased miR-449a and decreased Ki67 intensity. Our study highlighted the essential oncogenic properties of the SNHG7/miR-449a axis in pituitary adenoma.

Citing Articles

Regulation of long noncoding RNAs in the pathogenesis and clinical implications of pituitary adenomas.

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PMID: 37904679 PMC: 10571498. DOI: 10.1002/iid3.1047.

LncRNA SNHG7 Knockdown Aggravates Hepatic Ischemia-Reperfusion Injury and Promotes Apoptosis in Hemorrhagic Shock Pregnant Rats by Modulating miR-34a-5p/YWHAG Axis.

Han L, Tang J, Zhu S, Zhu J Mol Biotechnol. 2022; 65(6):983-996.

PMID: 36427097 DOI: 10.1007/s12033-022-00613-x.

Emerging Roles of miRNA, lncRNA, circRNA, and Their Cross-Talk in Pituitary Adenoma.

Wu W, Cao L, Jia Y, Xiao Y, Zhang X, Gui S Cells. 2022; 11(18).

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Long non-coding RNA-small nucleolar RNA host gene 7 regulates inflammatory responses following spinal cord injury by regulating the microRNA-449a/TNF-α-induced protein 3-interacting protein 2 axis.

He C, Xiao J, Ye Y, Huang S, Zhong Y, Liu L Bioengineered. 2022; 13(4):10215-10226.

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Silencing of long chain noncoding RNA paternally expressed gene (PEG10) inhibits the progression of neuroblastoma by regulating microRNA-449a (miR-449a)/ribosomal protein S2 (RPS2) axis.

Zhang J, Liu W, Ji P, Zhang Y Bioengineered. 2022; 13(3):6309-6322.

PMID: 35212607 PMC: 8973610. DOI: 10.1080/21655979.2022.2042999.

References

Bou Kheir T, Futoma-Kazmierczak E, Jacobsen A, Krogh A, Bardram L, Hother C . miR-449 inhibits cell proliferation and is down-regulated in gastric cancer. Mol Cancer. 2011; 10:29. PMC: 3070685. DOI: 10.1186/1476-4598-10-29. View

Buurman R, Gurlevik E, Schaffer V, Eilers M, Sandbothe M, Kreipe H . Histone deacetylases activate hepatocyte growth factor signaling by repressing microRNA-449 in hepatocellular carcinoma cells. Gastroenterology. 2012; 143(3):811-820.e15. DOI: 10.1053/j.gastro.2012.05.033. View

Chen Y, Peng Y, Xu Z, Ge B, Xiang X, Zhang T . Knockdown of lncRNA SNHG7 inhibited cell proliferation and migration in bladder cancer through activating Wnt/β-catenin pathway. Pathol Res Pract. 2018; 215(2):302-307. DOI: 10.1016/j.prp.2018.11.015. View

Ezzat S, Asa S, Couldwell W, Barr C, Dodge W, Vance M . The prevalence of pituitary adenomas: a systematic review. Cancer. 2004; 101(3):613-9. DOI: 10.1002/cncr.20412. View

Fang Y, Gu X, Li Z, Xiang J, Chen Z . miR-449b inhibits the proliferation of SW1116 colon cancer stem cells through downregulation of CCND1 and E2F3 expression. Oncol Rep. 2013; 30(1):399-406. DOI: 10.3892/or.2013.2465. View

Han Y, Hu H, Zhou J . Knockdown of LncRNA SNHG7 inhibited epithelial-mesenchymal transition in prostate cancer though miR-324-3p/WNT2B axis in vitro. Pathol Res Pract. 2019; 215(10):152537. DOI: 10.1016/j.prp.2019.152537. View

Huang S, Zheng S, Huang S, Cheng H, Lin Y, Wen Y . Flot2 targeted by miR-449 acts as a prognostic biomarker in glioma. Artif Cells Nanomed Biotechnol. 2019; 47(1):250-255. DOI: 10.1080/21691401.2018.1549062. View

Jang S, Yoo C, Park S, Kang S, Kim H . Low expression of miR-449 in gynecologic clear cell carcinoma. Int J Gynecol Cancer. 2014; 24(9):1558-63. DOI: 10.1097/IGC.0000000000000267. View

Jiang J, Yang X, He X, Ma W, Wang J, Zhou Q . MicroRNA-449b-5p suppresses the growth and invasion of breast cancer cells via inhibiting CREPT-mediated Wnt/β-catenin signaling. Chem Biol Interact. 2019; 302:74-82. DOI: 10.1016/j.cbi.2019.02.004. View

10.

Li L, Wu W, Sun D, Xie Z, Ma Y, Zhao Y . miR-449a and CDK6 in gastric carcinoma. Oncol Lett. 2014; 8(4):1533-1538. PMC: 4156198. DOI: 10.3892/ol.2014.2370. View

11.

Li Z, Huang X, Xu J, Su Q, Zhao J, Ma J . miR-449 overexpression inhibits papillary thyroid carcinoma cell growth by targeting RET kinase-β-catenin signaling pathway. Int J Oncol. 2016; 49(4):1629-1637. DOI: 10.3892/ijo.2016.3659. View

12.

Lin P, Liu X, Wang S, Li X, Song Y, Li L . Diagnosing pituitary adenoma in unstained sections based on multiphoton microscopy. Pituitary. 2018; 21(4):362-370. DOI: 10.1007/s11102-018-0882-6. View

13.

Luo W, Huang B, Li Z, Li H, Sun L, Zhang Q . MicroRNA-449a is downregulated in non-small cell lung cancer and inhibits migration and invasion by targeting c-Met. PLoS One. 2013; 8(5):e64759. PMC: 3667122. DOI: 10.1371/journal.pone.0064759. View

14.

Newey P, Thakker R . Role of multiple endocrine neoplasia type 1 mutational analysis in clinical practice. Endocr Pract. 2011; 17 Suppl 3:8-17. DOI: 10.4158/EP10379.RA. View

15.

Qi H, Wen B, Wu Q, Cheng W, Lou J, Wei J . Long noncoding RNA SNHG7 accelerates prostate cancer proliferation and cycle progression through cyclin D1 by sponging miR-503. Biomed Pharmacother. 2018; 102:326-332. DOI: 10.1016/j.biopha.2018.03.011. View

16.

Qu L, Ding J, Chen C, Wu Z, Liu B, Gao Y . Exosome-Transmitted lncARSR Promotes Sunitinib Resistance in Renal Cancer by Acting as a Competing Endogenous RNA. Cancer Cell. 2016; 29(5):653-668. DOI: 10.1016/j.ccell.2016.03.004. View

17.

Sun X, Huang T, Liu Z, Sun M, Luo S . LncRNA SNHG7 contributes to tumorigenesis and progression in breast cancer by interacting with miR-34a through EMT initiation and the Notch-1 pathway. Eur J Pharmacol. 2019; 856:172407. DOI: 10.1016/j.ejphar.2019.172407. View

18.

Wang C, Tan C, Wen Y, Zhang D, Li G, Chang L . FOXP1-induced lncRNA CLRN1-AS1 acts as a tumor suppressor in pituitary prolactinoma by repressing the autophagy via inactivating Wnt/β-catenin signaling pathway. Cell Death Dis. 2019; 10(7):499. PMC: 6591247. DOI: 10.1038/s41419-019-1694-y. View

19.

Wu Z, Yan L, Liu Y, Cao L, Guo Y, Zhang Y . Inhibition of mTORC1 by lncRNA H19 via disrupting 4E-BP1/Raptor interaction in pituitary tumours. Nat Commun. 2018; 9(1):4624. PMC: 6218470. DOI: 10.1038/s41467-018-06853-3. View

20.

Zhang H, Feng Z, Huang R, Xia Z, Xiang G, Zhang J . MicroRNA-449 suppresses proliferation of hepatoma cell lines through blockade lipid metabolic pathway related to SIRT1. Int J Oncol. 2014; 45(5):2143-52. DOI: 10.3892/ijo.2014.2596. View