Diagnosing Pituitary Adenoma in Unstained Sections Based on Multiphoton Microscopy

Overview

Journal Pituitary

Specialty Endocrinology

Date 2018 Mar 30

PMID 29594837

Citations 4

Authors

Peihua Lin

Xueyong Liu

Shu Wang

Xiaoling Li

Yankun Song

Lianhuang Li

Shanshan Cai

Xingfu Wang

Jianxin Chen

Affiliations

Soon will be listed here.

Abstract

Purpose: If we can find a new method that can achieve rapid diagnosis of adenoma during operation, it will help surgeon shorten the operation time and enhance the treatment efficacy. This study discusses the feasibility of multiphoton microscopy (MPM) in diagnosing pituitary adenoma.

Method: MPM, based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) is performed for the diagnosis of pituitary adenoma in unstained sections.

Results: Our results show that MPM can reveal the variation of reticulin fiber by SHG signals of collagen, combined with the measurement of area of acinus, thickness of collagen fiber and collagen percentage. MPM can further reflect the change of meshwork in normal pituitary and hyperplasia quantitatively. And the characteristics of typical growth patterns of pituitary adenoma are demonstrated by the overlay of SHG and TPEF images. What's more, we can identify the boundary of normal pituitary, hyperplasia and adenoma from MPM images. And the experiment also results verify the feasibility of this method in frozen sections.

Conclusion: These results indicated that MPM can make a diagnosis of pituitary adenoma by the morphological changes without routine pathological processing including hematoxylin-eosin (H&E) staining and other special staining. Therefore, this technique is expected to help diagnosis of pituitary adenoma during operation.

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References

Cushing H . III. Partial Hypophysectomy for Acromegaly: With Remarks on the Function of the Hypophysis. Ann Surg. 1909; 50(6):1002-17. PMC: 1407483. DOI: 10.1097/00000658-190912000-00003. View

Levene M, Dombeck D, Kasischke K, Molloy R, Webb W . In vivo multiphoton microscopy of deep brain tissue. J Neurophysiol. 2003; 91(4):1908-12. DOI: 10.1152/jn.01007.2003. View

Jarzembowski J, Lloyd R, McKeever P . Type IV collagen immunostaining is a simple, reliable diagnostic tool for distinguishing between adenomatous and normal pituitary glands. Arch Pathol Lab Med. 2007; 131(6):931-5. DOI: 10.5858/2007-131-931-TICIIA. View

Velasco M, Sindely S, ROESSMANN U . Reticulum stain for frozen-section diagnosis of pituitary adenomas. Technical note. J Neurosurg. 1977; 46(4):548-50. DOI: 10.3171/jns.1977.46.4.0548. View

Amirsolaimani B, Cromey B, Peyghambarian N, Kieu K . All-reflective multiphoton microscope. Opt Express. 2017; 25(19):23399-23407. DOI: 10.1364/OE.25.023399. View

Hirsch W, Zumkeller W, Teichler H, Jassoy A, Schluter A, Langer T . Microadenomas of the pituitary gland in children with and without hypophyseal dysfunction in magnetic resonance imaging. J Pediatr Endocrinol Metab. 2002; 15(2):157-62. DOI: 10.1515/JPEM.2002.15.2.157. View

Thomas R, Shenoy K, Seshadri M, Muliyil J, Rao A, Paul P . Visual field defects in non-functioning pituitary adenomas. Indian J Ophthalmol. 2002; 50(2):127-30. View

McLaughlin S, Ochoa-Frongia L, Patil S, Cody 3rd H, Sclafani L . Influence of frozen-section analysis of sentinel lymph node and lumpectomy margin status on reoperation rates in patients undergoing breast-conservation therapy. J Am Coll Surg. 2007; 206(1):76-82. DOI: 10.1016/j.jamcollsurg.2007.07.021. View

Jiang L, Wang X, Wu Z, Lin P, Du H, Wang S . Label-free detection of fibrillar collagen deposition associated with vascular elements in glioblastoma multiforme by using multiphoton microscopy. J Microsc. 2016; 265(2):207-213. DOI: 10.1111/jmi.12476. View

10.

Sun T, Haberman A, Greco V . Preclinical Advances with Multiphoton Microscopy in Live Imaging of Skin Cancers. J Invest Dermatol. 2016; 137(2):282-287. PMC: 5258832. DOI: 10.1016/j.jid.2016.08.033. View

11.

Horvath E, Kovacs K, Scheithauer B . Pituitary hyperplasia. Pituitary. 2000; 1(3-4):169-79. DOI: 10.1023/a:1009952930425. View

12.

Wu X, Chen G, Lu J, Zhu W, Qiu J, Chen J . Label-free detection of breast masses using multiphoton microscopy. PLoS One. 2013; 8(6):e65933. PMC: 3675049. DOI: 10.1371/journal.pone.0065933. View

13.

Kim B, Eichler J, Silva L . Frequency doubling of ultrashort laser pulses in biological tissues. Appl Opt. 2008; 38(34):7145-50. DOI: 10.1364/ao.38.007145. View

14.

Balu M, Mikami H, Hou J, Potma E, Tromberg B . Rapid mesoscale multiphoton microscopy of human skin. Biomed Opt Express. 2016; 7(11):4375-4387. PMC: 5119580. DOI: 10.1364/BOE.7.004375. View

15.

Konig K, Riemann I . High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. J Biomed Opt. 2003; 8(3):432-9. DOI: 10.1117/1.1577349. View

16.

Xu J, Kang D, Zeng Y, Zhuo S, Zhu X, Jiang L . Multiphoton microscopy for label-free identification of intramural metastasis in human esophageal squamous cell carcinoma. Biomed Opt Express. 2017; 8(7):3360-3368. PMC: 5508833. DOI: 10.1364/BOE.8.003360. View

17.

NAOUMENKO J, FEIGIN I . A simple silver solution for staining reticulin. Stain Technol. 1974; 49(3):153-5. DOI: 10.3109/10520297409116967. View

18.

Wu Y, Fu F, Lian Y, Chen J, Wang C, Nie Y . Monitoring morphological alterations during invasive ductal breast carcinoma progression using multiphoton microscopy. Lasers Med Sci. 2015; 30(3):1109-15. DOI: 10.1007/s10103-015-1712-y. View

19.

Mete O, Asa S . Clinicopathological correlations in pituitary adenomas. Brain Pathol. 2012; 22(4):443-53. PMC: 8029283. DOI: 10.1111/j.1750-3639.2012.00599.x. View

20.

Noh S, Kim S, Cho N, Kim S . Rapid Reticulin Fiber Staining Method is Helpful for the Diagnosis of Pituitary Adenoma in Frozen Section. Endocr Pathol. 2015; 26(2):178-84. DOI: 10.1007/s12022-015-9370-y. View