Alpha 2.0

» Articles » PMID: 32871750

Indocyanine Green Labels an Orthotopic Nude-Mouse Model of Very-Early Colon-Cancer Liver Metastases

Overview

Overview

Journal In Vivo

Specialty Oncology

Date 2020 Sep 3

PMID 32871750

Citations 5

Authors

Authors

Yoshihiko Tashiro

Hannah M Hollandsworth

Hiroto Nishino

Jun Yamamoto

Siamak Amirfakhri

Filemoni Filemoni

Norihiko Sugisawa

Takeshi Aoki

Masahiko Murakami

Robert M Hoffman

Michael Bouvet

Affiliations

Affiliations

Soon will be listed here.

Abstract

Background/aim: Near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) is used to visualize colon-cancer liver metastases (CCLM) during surgery. The present study aimed to use ICG to visualize small CCLM in an orthotopic mouse model.

Materials And Methods: A 3-mm fragment of HT29 human colon cancer was implanted to the liver of 5 athymic nude mice (nu/nu). The Pearl Trilogy Small Animal Fluorescence Imaging system was used 24 h after intravenous (IV) injection of 0.025 mg (0.25 mg/ml) ICG.

Results: In four of the five mice, tumor fluorescence was detected. Small tumors (approximately 3 mm) were distinctly visualized with a minimal fluorescence liver signal, with a mean tumor to liver signal ratio of 1.81 (SD±0.167).

Conclusion: Small CCLM can be clearly identified by ICG fluorescence in an orthotopic nude-mouse model. This model is translatable to the clinic for the detection of occult early CCLM.

Citing Articles

Near-infrared Fluorescence Imaging for Detecting Pancreatic Liver Metastasis in an Orthotopic Athymic Mouse Model.

Lee L, Hering N, Zibell M, Lobbes L, Kamphues C, Lauscher J In Vivo. 2023; 37(2):519-523.

PMID: 36881105 PMC: 10026647. DOI: 10.21873/invivo.13109.

Distribution of Embryonic Stem Cell-Derived Mesenchymal Stem Cells after Intravenous Infusion in Hypoxic-Ischemic Encephalopathy.

Lee S, Choung J, Kim J, Kim H, Kim M Life (Basel). 2023; 13(1).

PMID: 36676176 PMC: 9861288. DOI: 10.3390/life13010227.

A Novel Color-Coded Liver Metastasis Mouse Model to Distinguish Tumor and Adjacent Liver Segment.

Nishino H, Hollandsworth H, Amirfakhri S, Tashiro Y, Yamamoto J, Turner M J Surg Res. 2021; 264:327-333.

PMID: 33848831 PMC: 8222089. DOI: 10.1016/j.jss.2021.02.022.

Diagnostic imaging in near-infrared photoimmunotherapy using a commercially available camera for indocyanine green.

Inagaki F, Fujimura D, Furusawa A, Okada R, Wakiyama H, Kato T Cancer Sci. 2021; 112(3):1326-1330.

PMID: 33543819 PMC: 7935778. DOI: 10.1111/cas.14809.

Ligation Method to Specifically Label a Liver Segment With Indocyanine Green in an Orthotopic Nude-Mouse Liver-Metastasis Model.

Nishino H, Hollandsworth H, Tashiro Y, Yamamoto J, Amirfakhri S, Filemoni F In Vivo. 2020; 34(6):3159-3162.

PMID: 33144419 PMC: 7811678. DOI: 10.21873/invivo.12150.

References

1.

Aoki T, Murakami M, Koizumi T, Matsuda K, Fujimori A, Kusano T . Determination of the surgical margin in laparoscopic liver resections using infrared indocyanine green fluorescence. Langenbecks Arch Surg. 2018; 403(5):671-680. DOI: 10.1007/s00423-018-1685-y. View

2.

Oshiro H, Tome Y, Kiyuna T, Yoon S, Lwin T, Han Q . Oral Recombinant Methioninase Overcomes Colorectal-cancer Liver Metastasis Resistance to the Combination of 5-Fluorouracil and Oxaliplatinum in a Patient-derived Orthotopic Xenograft Mouse Model. Anticancer Res. 2019; 39(9):4667-4671. DOI: 10.21873/anticanres.13648. View

3.

Hoffman R . Patient-derived orthotopic xenografts: better mimic of metastasis than subcutaneous xenografts. Nat Rev Cancer. 2015; 15(8):451-2. DOI: 10.1038/nrc3972. View

4.

Hazhirkarzar B, Khoshpouri P, Shaghaghi M, Ghasabeh M, Pawlik T, Kamel I . Current state of the art imaging approaches for colorectal liver metastasis. Hepatobiliary Surg Nutr. 2020; 9(1):35-48. PMC: 7026783. DOI: 10.21037/hbsn.2019.05.11. View

5.

Hoffman R . Orthotopic metastatic mouse models for anticancer drug discovery and evaluation: a bridge to the clinic. Invest New Drugs. 2000; 17(4):343-59. DOI: 10.1023/a:1006326203858. View

6.

Morita Y, Sakaguchi T, Unno N, Shibasaki Y, Suzuki A, Fukumoto K . Detection of hepatocellular carcinomas with near-infrared fluorescence imaging using indocyanine green: its usefulness and limitation. Int J Clin Oncol. 2011; 18(2):232-41. DOI: 10.1007/s10147-011-0367-3. View

7.

Aoki T, Yasuda D, Shimizu Y, Odaira M, Niiya T, Kusano T . Image-guided liver mapping using fluorescence navigation system with indocyanine green for anatomical hepatic resection. World J Surg. 2008; 32(8):1763-7. DOI: 10.1007/s00268-008-9620-y. View

8.

Sahani D, Kalva S, Tanabe K, Hayat S, ONeill M, Halpern E . Intraoperative US in patients undergoing surgery for liver neoplasms: comparison with MR imaging. Radiology. 2004; 232(3):810-4. DOI: 10.1148/radiol.2323030896. View

9.

Hoffman R . Orthotopic is orthodox: why are orthotopic-transplant metastatic models different from all other models?. J Cell Biochem. 1994; 56(1):1-3. DOI: 10.1002/jcb.240560102. View

10.

Nomura K, Kadoya M, Ueda K, Fujinaga Y, Miwa S, Miyagawa S . Detection of hepatic metastases from colorectal carcinoma: comparison of histopathologic features of anatomically resected liver with results of preoperative imaging. J Clin Gastroenterol. 2007; 41(8):789-95. DOI: 10.1097/01.mcg.0000225676.22218.08. View

11.

Kuo T, Kubota T, Watanabe M, Furukawa T, Teramoto T, ISHIBIKI K . Liver colonization competence governs colon cancer metastasis. Proc Natl Acad Sci U S A. 1995; 92(26):12085-9. PMC: 40301. DOI: 10.1073/pnas.92.26.12085. View

12.

Fu X, Besterman J, Monosov A, Hoffman R . Models of human metastatic colon cancer in nude mice orthotopically constructed by using histologically intact patient specimens. Proc Natl Acad Sci U S A. 1991; 88(20):9345-9. PMC: 52711. DOI: 10.1073/pnas.88.20.9345. View

13.

Ishizawa T, Tamura S, Masuda K, Aoki T, Hasegawa K, Imamura H . Intraoperative fluorescent cholangiography using indocyanine green: a biliary road map for safe surgery. J Am Coll Surg. 2009; 208(1):e1-4. DOI: 10.1016/j.jamcollsurg.2008.09.024. View

14.

Yang M, Reynoso J, Bouvet M, Hoffman R . A transgenic red fluorescent protein-expressing nude mouse for color-coded imaging of the tumor microenvironment. J Cell Biochem. 2008; 106(2):279-84. PMC: 2739131. DOI: 10.1002/jcb.21999. View

15.

van der Vorst J, Hutteman M, Mieog J, de Rooij K, Kaijzel E, Lowik C . Near-infrared fluorescence imaging of liver metastases in rats using indocyanine green. J Surg Res. 2011; 174(2):266-71. PMC: 3138836. DOI: 10.1016/j.jss.2011.01.009. View

16.

Leen E, Ceccotti P, Moug S, Glen P, MacQuarrie J, Angerson W . Potential value of contrast-enhanced intraoperative ultrasonography during partial hepatectomy for metastases: an essential investigation before resection?. Ann Surg. 2006; 243(2):236-40. PMC: 1448920. DOI: 10.1097/01.sla.0000197708.77063.07. View

17.

Ishizawa T, Fukushima N, Shibahara J, Masuda K, Tamura S, Aoki T . Real-time identification of liver cancers by using indocyanine green fluorescent imaging. Cancer. 2009; 115(11):2491-504. DOI: 10.1002/cncr.24291. View

18.

Gotoh K, Yamada T, Ishikawa O, Takahashi H, Eguchi H, Yano M . A novel image-guided surgery of hepatocellular carcinoma by indocyanine green fluorescence imaging navigation. J Surg Oncol. 2009; 100(1):75-9. DOI: 10.1002/jso.21272. View