CRISPR/Cas13-Based Approaches for Ultrasensitive and Specific Detection of MicroRNAs

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2021 Aug 7

PMID 34359825

Citations 11

Authors

Javier T Granados-Riveron

Guillermo Aquino-Jarquin

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) have a prominent role in virtually every aspect of cell biology. Due to the small size of mature miRNAs, the high degree of similarity between miRNA family members, and the low abundance of miRNAs in body fluids, miRNA expression profiling is technically challenging. Biosensors based on electrochemical detection for nucleic acids are a novel category of inexpensive and very sensitive diagnostic tools. On the other hand, after recognizing the target sequence, specific CRISPR-associated proteins, including orthologues of Cas12, Cas13, and Cas14, exhibit collateral nonspecific catalytic activities that can be employed for specific and ultrasensitive nucleic acid detection from clinically relevant samples. Recently, several platforms have been developed, connecting the benefits of enzyme-assisted signal amplification and enzyme-free amplification biosensing technologies with CRISPR-based approaches for miRNA detection. Together, they provide high sensitivity, precision, and fewer limitations in diagnosis through efficient sensors at a low cost and a simple miniaturized readout. This review provides an overview of several CRISPR-based biosensing platforms that have been developed and successfully applied for ultrasensitive and specific miRNA detection.

Citing Articles

Exonuclease-III Assisted Signal Cycle Integrating with Self-Priming Mediated Chain Extension for Sensitive and Reliable MicroRNA Detection.

Li C, Zheng X, Xie S, Lin D ACS Omega. 2025; 10(6):6228-6233.

PMID: 39989823 PMC: 11840618. DOI: 10.1021/acsomega.4c11417.

CRISPR-Cas13a-Triggered DNAzyme Signal Amplification-Based Colorimetric miRNA Detection Method and Its Application in Evaluating the Anxiety.

Yan N, Hu Z, Zhang L Appl Biochem Biotechnol. 2024; 196(11):7896-7907.

PMID: 38652440 DOI: 10.1007/s12010-024-04951-1.

CRISPR/Cas14 and G-Quadruplex DNAzyme-Driven Biosensor for Paper-Based Colorimetric Detection of African Swine Fever Virus.

Zhao X, He Y, Shao S, Ci Q, Chen L, Lu X ACS Sens. 2024; 9(5):2413-2420.

PMID: 38635911 PMC: 11216275. DOI: 10.1021/acssensors.4c00090.

A highly sensitive colorimetric DNA sensor for MicroRNA-155 detection: leveraging the peroxidase-like activity of copper nanoparticles in a double amplification strategy.

El Aamri M, Mohammadi H, Amine A Mikrochim Acta. 2023; 191(1):32.

PMID: 38102528 DOI: 10.1007/s00604-023-06087-1.

New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity.

Molina Vargas A, Sinha S, Osborn R, Arantes P, Patel A, Dewhurst S Nucleic Acids Res. 2023; 52(2):921-939.

PMID: 38033324 PMC: 10810210. DOI: 10.1093/nar/gkad1132.

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