Rapid MicroRNA Detection Using Power-free Microfluidic Chip: Coaxial Stacking Effect Enhances the Sandwich Hybridization

Overview

Journal Analyst

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2012 May 23

PMID 22614070

Citations 18

Authors

Hideyuki Arata

Hiroshi Komatsu

Aishan Han

Kazuo Hosokawa

Mizuo Maeda

Affiliations

Soon will be listed here.

Abstract

We present a new method for rapid microRNA detection with a small volume of sample using the power-free microfluidic device driven by degassed PDMS. Target microRNA was detected by sandwich hybridization taking advantage of the coaxial stacking effect. This method allows us to detect miR-21 in 20 min with a 0.5 μL sample volume at a limit of detection of 0.62 nM. Since microRNAs can act as cancer markers, this method might substantially contribute to future point-of-care cancer diagnosis.

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