Micromirror-Embedded Coverslip Assembly for Bidirectional Microscopic Imaging

Overview

Journal Micromachines (Basel)

Publisher MDPI

Date 2020 Jun 14

PMID 32532128

Citations 2

Authors

Dongwoo Lee

Jihye Kim

Eunjoo Song

Ji-Young Jeong

Eun-Chae Jeon

Pilhan Kim

Wonhee Lee

Affiliations

Soon will be listed here.

Abstract

3D imaging of a biological sample provides information about cellular and subcellular structures that are important in cell biology and related diseases. However, most 3D imaging systems, such as confocal and tomographic microscopy systems, are complex and expensive. Here, we developed a quasi-3D imaging tool that is compatible with most conventional microscopes by integrating micromirrors and microchannel structures on coverslips to provide bidirectional imaging. Microfabricated micromirrors had a precisely 45° reflection angle and optically clean reflective surfaces with high reflectance over 95%. The micromirrors were embedded on coverslips that could be assembled as a microchannel structure. We demonstrated that this simple disposable device allows a conventional microscope to perform bidirectional imaging with simple control of a focal plane. Images of microbeads and cells under bright-field and fluorescent microscopy show that the device can provide a quick analysis of 3D information, such as 3D positions and subcellular structures.

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