Optical Diffraction Tomography for High Resolution Live Cell Imaging

Overview

Journal Opt Express

Specialties Biophysics
Ophthalmology

Date 2009 Jan 9

PMID 19129896

Citations 116

Authors

Yongjin Sung

Wonshik Choi

Christopher Fang-Yen

Kamran Badizadegan

Ramachandra R Dasari

Michael S Feld

Affiliations

Soon will be listed here.

Abstract

We report the experimental implementation of optical diffraction tomography for quantitative 3D mapping of refractive index in live biological cells. Using a heterodyne Mach-Zehnder interferometer, we record complex field images of light transmitted through a sample with varying directions of illumination. To quantitatively reconstruct the 3D map of complex refractive index in live cells, we apply optical diffraction tomography based on the Rytov approximation. In this way, the effect of diffraction is taken into account in the reconstruction process and diffraction-free high resolution 3D images are obtained throughout the entire sample volume. The quantitative refractive index map can potentially serve as an intrinsic assay to provide the molecular concentrations without the addition of exogenous agents and also to provide a method for studying the light scattering properties of single cells.

Citing Articles

Multi-modal transport of intensity diffraction tomography microscopy with an electrically tunable lens [Invited].

Zhou Z, Zhang R, Zhou N, Chen Q, Zuo C Biomed Opt Express. 2025; 16(2):837-848.

PMID: 39958838 PMC: 11828454. DOI: 10.1364/BOE.545258.

Label-free imaging flow cytometry for cell classification based directly on multiple off-axis holographic projections.

Aharoni D, Dudaie M, Barnea I, Shaked N J Biomed Opt. 2025; 30(1):016007.

PMID: 39850327 PMC: 11754690. DOI: 10.1117/1.JBO.30.1.016007.

From 3D to 2D and back again.

Dinc N, Saba A, Madrid-Wolff J, Gigli C, Boniface A, Moser C Nanophotonics. 2024; 12(5):777-793.

PMID: 39634355 PMC: 11501230. DOI: 10.1515/nanoph-2022-0512.

DMD and microlens array as a switchable module for illumination angle scanning in optical diffraction tomography.

Yang S, Kim J, Swartz M, Eberhart J, Chowdhury S Biomed Opt Express. 2024; 15(10):5932-5946.

PMID: 39421770 PMC: 11482169. DOI: 10.1364/BOE.535123.

Ultrabroadband Optical Diffraction Tomography.

Hormann M, Camargo F, van Hulst N, Cerullo G, Liebel M ACS Photonics. 2024; 11(9):3680-3687.

PMID: 39310293 PMC: 11413850. DOI: 10.1021/acsphotonics.4c00797.

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