Label-free Characterization of White Blood Cells by Measuring 3D Refractive Index Maps

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2015 Oct 28

PMID 26504637

Citations 42

Authors

Jonghee Yoon

Kyoohyun Kim

Hyunjoo Park

Chulhee Choi

Seongsoo Jang

YongKeun Park

Affiliations

Soon will be listed here.

Abstract

The characterization of white blood cells (WBCs) is crucial for blood analyses and disease diagnoses. However, current standard techniques rely on cell labeling, a process which imposes significant limitations. Here we present three-dimensional (3D) optical measurements and the label-free characterization of mouse WBCs using optical diffraction tomography. 3D refractive index (RI) tomograms of individual WBCs are constructed from multiple two-dimensional quantitative phase images of samples illuminated at various angles of incidence. Measurements of the 3D RI tomogram of WBCs enable the separation of heterogeneous populations of WBCs using quantitative morphological and biochemical information. Time-lapse tomographic measurements also provide the 3D trajectory of micrometer-sized beads ingested by WBCs. These results demonstrate that optical diffraction tomography can be a useful and versatile tool for the study of WBCs.

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References

Colomb T, Durr F, Cuche E, Marquet P, Limberger H, Salathe R . Polarization microscopy by use of digital holography: application to optical-fiber birefringence measurements. Appl Opt. 2005; 44(21):4461-9. DOI: 10.1364/ao.44.004461. View

Handwerger K, Cordero J, Gall J . Cajal bodies, nucleoli, and speckles in the Xenopus oocyte nucleus have a low-density, sponge-like structure. Mol Biol Cell. 2004; 16(1):202-11. PMC: 539164. DOI: 10.1091/mbc.e04-08-0742. View

Ekpenyong A, Man S, Achouri S, Bryant C, Guck J, Chalut K . Bacterial infection of macrophages induces decrease in refractive index. J Biophotonics. 2012; 6(5):393-7. DOI: 10.1002/jbio.201200113. View

Jourdain P, Pavillon N, Moratal C, Boss D, Rappaz B, Depeursinge C . Determination of transmembrane water fluxes in neurons elicited by glutamate ionotropic receptors and by the cotransporters KCC2 and NKCC1: a digital holographic microscopy study. J Neurosci. 2011; 31(33):11846-54. PMC: 6623187. DOI: 10.1523/JNEUROSCI.0286-11.2011. View

Byun H, Hillman T, Higgins J, Diez-Silva M, Peng Z, Dao M . Optical measurement of biomechanical properties of individual erythrocytes from a sickle cell patient. Acta Biomater. 2012; 8(11):4130-8. PMC: 3576574. DOI: 10.1016/j.actbio.2012.07.011. View

Ngkelo A, Meja K, Yeadon M, Adcock I, Kirkham P . LPS induced inflammatory responses in human peripheral blood mononuclear cells is mediated through NOX4 and Giα dependent PI-3kinase signalling. J Inflamm (Lond). 2012; 9(1):1. PMC: 3293082. DOI: 10.1186/1476-9255-9-1. View

Lee S, Kim Y, Lee J, Rhee J, Park C, Kim D . Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy. J Biomed Opt. 2011; 16(3):036004. DOI: 10.1117/1.3548882. View

Nieminen M, Henttinen T, Merinen M, Marttila-Ichihara F, Eriksson J, Jalkanen S . Vimentin function in lymphocyte adhesion and transcellular migration. Nat Cell Biol. 2006; 8(2):156-62. DOI: 10.1038/ncb1355. View

Zou W, Restifo N . T(H)17 cells in tumour immunity and immunotherapy. Nat Rev Immunol. 2010; 10(4):248-56. PMC: 3242804. DOI: 10.1038/nri2742. View

10.

Park H, Hong S, Kim K, Cho S, Lee W, Kim Y . Characterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy. Sci Rep. 2015; 5:10827. PMC: 4650620. DOI: 10.1038/srep10827. View

11.

Majhail N, Lichtin A . Acute leukemia with a very high leukocyte count: confronting a medical emergency. Cleve Clin J Med. 2004; 71(8):633-7. DOI: 10.3949/ccjm.71.8.633. View

12.

Chalut K, Ekpenyong A, Clegg W, Melhuish I, Guck J . Quantifying cellular differentiation by physical phenotype using digital holographic microscopy. Integr Biol (Camb). 2012; 4(3):280-4. DOI: 10.1039/c2ib00129b. View

13.

Kim K, Kim K, Park H, Ye J, Park Y . Real-time visualization of 3-D dynamic microscopic objects using optical diffraction tomography. Opt Express. 2014; 21(26):32269-78. DOI: 10.1364/OE.21.032269. View

14.

Kim Y, Shim H, Kim K, Park H, Jang S, Park Y . Profiling individual human red blood cells using common-path diffraction optical tomography. Sci Rep. 2014; 4:6659. PMC: 4200412. DOI: 10.1038/srep06659. View

15.

Ray A, Dittel B . Isolation of mouse peritoneal cavity cells. J Vis Exp. 2010; (35). PMC: 3152216. DOI: 10.3791/1488. View

16.

Judkewitz B, Wang Y, Horstmeyer R, Mathy A, Yang C . Speckle-scale focusing in the diffusive regime with time-reversal of variance-encoded light (TROVE). Nat Photonics. 2013; 7(4):300-305. PMC: 3692396. DOI: 10.1038/nphoton.2013.31. View

17.

Zangle T, Burnes D, Mathis C, Witte O, Teitell M . Quantifying biomass changes of single CD8+ T cells during antigen specific cytotoxicity. PLoS One. 2013; 8(7):e68916. PMC: 3720853. DOI: 10.1371/journal.pone.0068916. View

18.

Zangle T, Teitell M . Live-cell mass profiling: an emerging approach in quantitative biophysics. Nat Methods. 2014; 11(12):1221-8. PMC: 4319180. DOI: 10.1038/nmeth.3175. View

19.

Balagopalan L, Sherman E, Barr V, Samelson L . Imaging techniques for assaying lymphocyte activation in action. Nat Rev Immunol. 2010; 11(1):21-33. PMC: 3403683. DOI: 10.1038/nri2903. View

20.

Park Y, Best C, Badizadegan K, Dasari R, Feld M, Kuriabova T . Measurement of red blood cell mechanics during morphological changes. Proc Natl Acad Sci U S A. 2010; 107(15):6731-6. PMC: 2872375. DOI: 10.1073/pnas.0909533107. View