Genome Analysis of a Novel Tembusu Virus in Taiwan

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2020 May 28

PMID 32455871

Citations 13

Authors

Shih-Huan Peng

Chien-Ling Su

Mei-Chun Chang

Huai-Chin Hu

Su-Lin Yang

Pei-Yun Shu

Affiliations

Soon will be listed here.

Abstract

We identified and isolated a novel Tembusu virus (TMUV) strain TP1906 (TMUV-TP1906) from a mosquito pool collected from the northern part of Taiwan in 2019. The TMUV-TP1906 genome is a 10,990-nucleotide-long, positive-sense, single-stranded RNA, consisting of a single open reading frame (ORF) encoding a polyprotein of 3425 amino acids, with 5' and 3' untranslated regions (UTRs) of 94 and 618 nucleotides, respectively. The nucleotide sequence of the TMUV-TP1906 of ORF exhibited 93.71% and 91.27% similarity with Sitiawan virus (STWV) and the TMUV prototype strain MM1775, respectively. The 3'-UTR variable region of TMUV-TP1906 showed nucleotide sequence divergence with other TMUV strains. Phylogenetic analysis of the complete ORF and polyprotein sequences revealed that TMUV-TP1906 is most closely related to STWV which causes encephalitis and retarded growth in chickens. We found that the TMUV-TP1906 caused a cytopathic effect (CPE) in the DF-1 chicken fibroblast cell line, while no apparent CPE was observed in Vero and C6/36 cells. In this study, we first identified and isolated a novel TMUV strain in Taiwan. In addition, to our knowledge, it is the first time that the TMUV strain was isolated from the mosquitoes. Further study is warranted to investigate the host range and virulence of TMUV-TP1906.

Citing Articles

Complete coding sequence of Tembusu virus isolate 2211-5 isolated in Taiwan.

Chen Y, Liao C, Huang C, Lee F Microbiol Resour Announc. 2024; 13(11):e0048224.

PMID: 39315837 PMC: 11556066. DOI: 10.1128/mra.00482-24.

Advancements in Research on Duck Tembusu Virus Infections.

Cheng Y, Wang R, Wu Q, Chen J, Wang A, Wu Z Viruses. 2024; 16(5).

PMID: 38793692 PMC: 11126125. DOI: 10.3390/v16050811.

Epidemiology, genetic diversity, and evolutionary dynamics of Tembusu virus.

Zhou P, Ma B, Gao Y, Xu Y, Li Z, Jin H Arch Virol. 2023; 168(10):262.

PMID: 37773423 DOI: 10.1007/s00705-023-05885-5.

Complete coding sequence of Tembusu virus isolated from geese in Taiwan in 2020.

Chen Y, Huang C, Lee F Microbiol Resour Announc. 2023; 12(10):e0025623.

PMID: 37712679 PMC: 10586138. DOI: 10.1128/MRA.00256-23.

Identification of the Tembusu Virus in Mosquitoes in Northern Thailand.

Hamel R, Vargas R, Rajonhson D, Yamanaka A, Jaroenpool J, Wichit S Viruses. 2023; 15(7).

PMID: 37515135 PMC: 10385312. DOI: 10.3390/v15071447.

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