Persistence of Tembusu Virus in in Yunnan Province, China

Overview

Journal Pathogens

Date 2023 Mar 29

PMID 36986412

Authors

Danhe Hu

Chao Wu

Ruichen Wang

Xiaohui Yao

Kai Nie

Quan Lv

Shihong Fu

Qikai Yin

Wenzhe Su

Fan Li

Songtao Xu

Ying He

Guodong Liang

Xiangdong Li

Huanyu Wang

Affiliations

Soon will be listed here.

Abstract

The Tembusu virus (TMUV), a member of the Flaviviridae family, can be transmitted via mosquitoes and cause poultry disease. In 2020, a strain of TMUV (YN2020-20) was isolated from mosquito samples collected in Yunnan province, China. In vitro experiments showed that TMUV-YN2020-20 produced a significant cytopathic effect (CPE) in BHK, DF-1, and VERO cells, while the CPE in C6/36 cells was not significant. Phylogenetic analysis revealed that the strain belonged to Cluster 3.2 and was closely related to the Yunnan mosquito-derived isolates obtained in 2012 and the Shandong avian-derived isolate obtained in 2014. Notably, TMUV-YN2020-20 developed five novel mutations (E-V358I, NS1-Y/F/I113L, NS4A-T/A89V, NS4B-D/E/N/C22S, and NS5-E638G) at loci that were relatively conserved previously. The results of this study demonstrate the continuous circulation and unique evolution of TMUV in mosquitoes in Yunnan province and suggest that appropriate surveillance should be taken.

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