Large-scale Electron Microscopy Database for Human Type 1 Diabetes

Overview

Journal Nat Commun

Specialty Biology

Date 2020 May 20

PMID 32424134

Citations 39

Authors

Pascal de Boer

Nicole M Pirozzi

Anouk H G Wolters

Jeroen Kuipers

Irina Kusmartseva

Mark A Atkinson

Martha Campbell-Thompson

Ben N G Giepmans

Affiliations

Soon will be listed here.

Abstract

Autoimmune β-cell destruction leads to type 1 diabetes, but the pathophysiological mechanisms remain unclear. To help address this void, we created an open-access online repository, unprecedented in its size, composed of large-scale electron microscopy images ('nanotomy') of human pancreas tissue obtained from the Network for Pancreatic Organ donors with Diabetes (nPOD; www.nanotomy.org). Nanotomy allows analyses of complete donor islets with up to macromolecular resolution. Anomalies we found in type 1 diabetes included (i) an increase of 'intermediate cells' containing granules resembling those of exocrine zymogen and endocrine hormone secreting cells; and (ii) elevated presence of innate immune cells. These are our first results of mining the database and support recent findings that suggest that type 1 diabetes includes abnormalities in the exocrine pancreas that may induce endocrine cellular stress as a trigger for autoimmunity.

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