A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells Across Development and from Human Pluripotent Stem Cells

Overview

Journal Cell Stem Cell

Publisher Cell Press

Specialty Cell Biology

Date 2020 May 13

PMID 32396864

Citations 56

Authors

Haibin Xi

Justin Langerman

Shan Sabri

Peggie Chien

Courtney S Young

Shahab Younesi

Michael Hicks

Karen Gonzalez

Wakana Fujiwara

Julia Marzi

Simone Liebscher

Melissa Spencer

Ben Van Handel

Denis Evseenko

Katja Schenke-Layland

Kathrin Plath

April D Pyle

Affiliations

Soon will be listed here.

Abstract

The developmental trajectory of human skeletal myogenesis and the transition between progenitor and stem cell states are unclear. We used single-cell RNA sequencing to profile human skeletal muscle tissues from embryonic, fetal, and postnatal stages. In silico, we identified myogenic as well as other cell types and constructed a "roadmap" of human skeletal muscle ontogeny across development. In a similar fashion, we also profiled the heterogeneous cell cultures generated from multiple human pluripotent stem cell (hPSC) myogenic differentiation protocols and mapped hPSC-derived myogenic progenitors to an embryonic-to-fetal transition period. We found differentially enriched biological processes and discovered co-regulated gene networks and transcription factors present at distinct myogenic stages. This work serves as a resource for advancing our knowledge of human myogenesis. It also provides a tool for a better understanding of hPSC-derived myogenic progenitors for translational applications in skeletal muscle-based regenerative medicine.

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