Generation of Highly Pure Pluripotent Stem Cell-derived Myogenic Progenitor Cells and Myotubes

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2023 Dec 15

PMID 38101399

Authors

Reem Bou Akar

Cheryane Lama

Deborah Aubin

Julien Maruotti

Brigitte Onteniente

Joana Esteves de Lima

Frederic Relaix

Affiliations

Soon will be listed here.

Abstract

Driving efficient and pure skeletal muscle cell differentiation from pluripotent stem cells (PSCs) has been challenging. Here, we report an optimized protocol that generates skeletal muscle progenitor cells with high efficiency and purity in a short period of time. Human induced PSCs (hiPSCs) and murine embryonic stem cells (mESCs) were specified into the mesodermal myogenic fate using distinct and species-specific protocols. We used a specific maturation medium to promote the terminal differentiation of both human and mouse myoblast populations, and generated myotubes associated with a large pool of cell-cycle arrested PAX7 cells. We also show that myotube maturation is modulated by dish-coating properties, cell density, and percentage of myogenic progenitor cells. Given the high efficiency in the generation of myogenic progenitors and differentiated myofibers, this protocol provides an attractive strategy for tissue engineering, modeling of muscle dystrophies, and evaluation of new therapeutic approaches in vitro.

Citing Articles

Multitasking muscle: engineering iPSC-derived myogenic progenitors to do more.

Hamer M, Rossi F Front Cell Dev Biol. 2025; 12:1526635.

PMID: 39911186 PMC: 11794491. DOI: 10.3389/fcell.2024.1526635.

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