IKZF3/Aiolos Is Associated with but Not Sufficient for the Expression of IL-10 by CD4 T Cells

Overview

Journal J Immunol

Specialty Allergy & Immunology

Date 2020 Apr 24

PMID 32321757

Citations 14

Authors

Michael L Ridley

Veerle Fleskens

Ceri A Roberts

Sylvine Lalnunhlimi

Aldana Alnesf

Aoife M OByrne

Kathryn J A Steel

Giovanni A M Povoleri

Jonathan Sumner

Paul Lavender

Leonie S Taams

Affiliations

Soon will be listed here.

Abstract

The expression of anti-inflammatory IL-10 by CD4 T cells is indispensable for immune homeostasis, as it allows T cells to moderate their effector function. We previously showed that TNF-α blockade during T cell stimulation in CD4 T cell/monocyte cocultures resulted in maintenance of IL-10-producing T cells and identified IKZF3 as a putative regulator of IL-10. In this study, we tested the hypothesis that IKZF3 is a transcriptional regulator of IL-10 using a human CD4 T cell-only culture system. IL-10 CD4 T cells expressed the highest levels of IKZF3 both ex vivo and after activation compared with IL-10-CD4 T cells. Pharmacological targeting of IKZF3 with the drug lenalidomide showed that IKZF3 is required for anti-CD3/CD28 mAb-mediated induction of IL-10 but is dispensable for ex vivo IL-10 expression. However, overexpression of IKZF3 was unable to upregulate IL-10 at the mRNA or protein level in CD4 T cells and did not drive the transcription of the promoter or putative local enhancer constructs. Collectively, these data indicate that IKZF3 is associated with but not sufficient for IL-10 expression in CD4 T cells.

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