Anti-cancer Properties of Potential Probiotics and Their Cell-free Supernatants for the Prevention of Colorectal Cancer: an In Vitro Study

Overview

Journal Probiotics Antimicrob Proteins

Publisher Springer

Specialties Infectious Diseases
Microbiology
Nutritional Sciences
Pharmacology

Date 2022 Jul 27

PMID 35895217

Authors

Nuntiya Pahumunto

Rawee Teanpaisan

Affiliations

Soon will be listed here.

Abstract

This study aimed to characterize the anti-cancer properties of potential probiotics (Lacticaseibacillus paracasei SD1, Lacticaseibacillus rhamnosus SD4, Lacticaseibacillus rhamnosus SD11, and Lacticaseibacillus rhamnosus GG) and their cell-free supernatants (CFS) for the prevention of colorectal cancer (CRC), which including anti-bacterial and anti-inflammation activities against pathogens associated with CRC (Fusobacterium nucleatum, Porphyromonas gingivalis, ETEC, and Salmonella enterica). The expression of human β-defensin (2-4) and IL-10 after being stimulated with probiotics was also examined. In addition, anti-cancer activity of CFS and probiotic growth under intestinal conditions were determined. An in vitro study was conducted in the Caco-2 and HIEC-6 cells. Results showed that probiotic cells and their CFS displayed different antibacterial activity, and L. rhamnosus SD11 showed the strongest inhibition of the growth of pathogens. Additionally, both probiotic cell walls and their CFS suppressed pro-inflammatory cytokines after being stimulated with pathogens in Caco-2 and HIEC-6 cells. L. paracasei SD1 and L. rhamnosus SD11 showed significantly higher suppression levels than others and also both strains can stimulate highly expression of hBD (2-4) and IL-10. The CFS of L. paracasei SD1 and L. rhamnosus SD11 inhibited significantly high growth of Caco-2 cells but not much in HIEC-6 cells. Furthermore, all probiotics adhered to Caco-2 and HIEC-6 cells, and L. rhamnosus SD4 showed the highest adhesion to both cells. They could survive more than 70% in intestinal conditions. In conclusion, results indicate that potential probiotics tested exhibited various anti-cancer properties, which may be good candidates used as biotherapy for the prevention or to delay the progression of CRC.

Citing Articles

Anticancer activity of Weizmannia coagulans MZY531on H22 tumor-bearing mice by regulating inflammation, autophagy-dependent apoptosis, and gut microbiota.

Liu C, Tan M, Zhao L, Gai M, Zhou T, Yu C Sci Rep. 2025; 15(1):8250.

PMID: 40065050 PMC: 11894224. DOI: 10.1038/s41598-025-92825-9.

Cell free supernatants of Bifidobacterium adolescentis and Bifidobacterium longum suppress the tumor growth in colorectal cancer organoid model.

Kim M, Song M, Ji Y, Park J, Shin Y, Kim S Sci Rep. 2025; 15(1):935.

PMID: 39762302 PMC: 11704243. DOI: 10.1038/s41598-024-83048-5.

Investigating Chemical Composition and Functionality of Lactobacillus acidophilus LA-5 Postbiotics Prepared in Classic and Cheese Whey Media.

Nasri F, Alizadeh A, Kursad Incili G, Hayaloglu A, Moradi M Probiotics Antimicrob Proteins. 2024; .

PMID: 39693008 DOI: 10.1007/s12602-024-10435-9.

Appraisal of postbiotics in cancer therapy.

Sudaarsan A, Ghosh A Front Pharmacol. 2024; 15:1436021.

PMID: 39372197 PMC: 11449718. DOI: 10.3389/fphar.2024.1436021.

Postbiotics are a candidate for new functional foods.

Wei L, Wang B, Bai J, Zhang Y, Liu C, Suo H Food Chem X. 2024; 23:101650.

PMID: 39113733 PMC: 11304867. DOI: 10.1016/j.fochx.2024.101650.

References

Wong S, Yu J . Gut microbiota in colorectal cancer: mechanisms of action and clinical applications. Nat Rev Gastroenterol Hepatol. 2019; 16(11):690-704. DOI: 10.1038/s41575-019-0209-8. View

Schlee M, Wehkamp J, Altenhoefer A, Oelschlaeger T, Stange E, Fellermann K . Induction of human beta-defensin 2 by the probiotic Escherichia coli Nissle 1917 is mediated through flagellin. Infect Immun. 2007; 75(5):2399-407. PMC: 1865783. DOI: 10.1128/IAI.01563-06. View

Sophatha B, Piwat S, Teanpaisan R . Adhesion, anti-adhesion and aggregation properties relating to surface charges of selected Lactobacillus strains: study in Caco-2 and H357 cells. Arch Microbiol. 2020; 202(6):1349-1357. DOI: 10.1007/s00203-020-01846-7. View

Pahumunto N, Piwat S, Chanvitan S, Ongwande W, Uraipan S, Teanpaisan R . Fermented milk containing a potential probiotic SD11 with maltitol reduces : A double-blind, randomized, controlled study. J Dent Sci. 2021; 15(4):403-410. PMC: 7816037. DOI: 10.1016/j.jds.2020.03.003. View

Manmontri C, Nirunsittirat A, Piwat S, Wattanarat O, Pahumunto N, Makeudom A . Reduction of Streptococcus mutans by probiotic milk: a multicenter randomized controlled trial. Clin Oral Investig. 2019; 24(7):2363-2374. DOI: 10.1007/s00784-019-03095-5. View

Wattanarat O, Nirunsittirat A, Piwat S, Manmontri C, Teanpaisan R, Pahumunto N . Significant elevation of salivary human neutrophil peptides 1-3 levels by probiotic milk in preschool children with severe early childhood caries: a randomized controlled trial. Clin Oral Investig. 2020; 25(5):2891-2903. DOI: 10.1007/s00784-020-03606-9. View

Teanpaisan R, Dahlen G . Use of polymerase chain reaction techniques and sodium dodecyl sulfate-polyacrylamide gel electrophoresis for differentiation of oral Lactobacillus species. Oral Microbiol Immunol. 2006; 21(2):79-83. DOI: 10.1111/j.1399-302X.2006.00259.x. View

Fukushima H, Tsunomori Y, Seki R . Duplex real-time SYBR green PCR assays for detection of 17 species of food- or waterborne pathogens in stools. J Clin Microbiol. 2003; 41(11):5134-46. PMC: 262470. DOI: 10.1128/JCM.41.11.5134-5146.2003. View

Saygun I, Kubar A, Sahin S, Sener K, Slots J . Quantitative analysis of association between herpesviruses and bacterial pathogens in periodontitis. J Periodontal Res. 2007; 43(3):352-9. DOI: 10.1111/j.1600-0765.2007.01043.x. View

10.

Morillo J, Lau L, Sanz M, Herrera D, Silva A . Quantitative real-time PCR based on single copy gene sequence for detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. J Periodontal Res. 2003; 38(5):518-24. DOI: 10.1034/j.1600-0765.2003.00684.x. View

11.

Pahumunto N, Chotjumlong P, Makeudom A, Krisanaprakornkit S, Dahlen G, Teanpaisan R . Pro-inflammatory cytokine responses in human gingival epithelial cells after stimulation with cell wall extract of Aggregatibacter actinomycetemcomitans subtypes. Anaerobe. 2017; 48:103-109. DOI: 10.1016/j.anaerobe.2017.08.001. View

12.

Krisanaprakornkit S, Kimball J, Weinberg A, Darveau R, Bainbridge B, Dale B . Inducible expression of human beta-defensin 2 by Fusobacterium nucleatum in oral epithelial cells: multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier. Infect Immun. 2000; 68(5):2907-15. PMC: 97503. DOI: 10.1128/IAI.68.5.2907-2915.2000. View

13.

Fang X, Shu Q, Chen Q, Book M, Sahl H, Hoeft A . Differential expression of alpha- and beta-defensins in human peripheral blood. Eur J Clin Invest. 2002; 33(1):82-7. DOI: 10.1046/j.1365-2362.2003.01076.x. View

14.

Ridley M, Fleskens V, Roberts C, Lalnunhlimi S, Alnesf A, OByrne A . IKZF3/Aiolos Is Associated with but Not Sufficient for the Expression of IL-10 by CD4 T Cells. J Immunol. 2020; 204(11):2940-2948. PMC: 7231851. DOI: 10.4049/jimmunol.1901283. View

15.

Piwat S, Sophatha B, Teanpaisan R . An assessment of adhesion, aggregation and surface charges of Lactobacillus strains derived from the human oral cavity. Lett Appl Microbiol. 2015; 61(1):98-105. DOI: 10.1111/lam.12434. View

16.

Charteris W, Kelly P, Morelli L, Collins J . Development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic Lactobacillus and Bifidobacterium species in the upper human gastrointestinal tract. J Appl Microbiol. 1998; 84(5):759-68. DOI: 10.1046/j.1365-2672.1998.00407.x. View

17.

Pumriw S, Luang-In V, Samappito W . Screening of Probiotic Lactic Acid Bacteria Isolated from Fermented Pak-Sian for Use as a Starter Culture. Curr Microbiol. 2021; 78(7):2695-2707. DOI: 10.1007/s00284-021-02521-w. View

18.

Eaden J, Abrams K, Mayberry J . The risk of colorectal cancer in ulcerative colitis: a meta-analysis. Gut. 2001; 48(4):526-35. PMC: 1728259. DOI: 10.1136/gut.48.4.526. View

19.

Canavan C, Abrams K, Mayberry J . Meta-analysis: colorectal and small bowel cancer risk in patients with Crohn's disease. Aliment Pharmacol Ther. 2006; 23(8):1097-104. DOI: 10.1111/j.1365-2036.2006.02854.x. View

20.

Wu Y, Zhu C, Chen Z, Chen Z, Zhang W, Ma X . Protective effects of Lactobacillus plantarum on epithelial barrier disruption caused by enterotoxigenic Escherichia coli in intestinal porcine epithelial cells. Vet Immunol Immunopathol. 2016; 172:55-63. DOI: 10.1016/j.vetimm.2016.03.005. View