Stem Rust Resistance in Wheat is Suppressed by a Subunit of the Mediator Complex

Overview

Journal Nat Commun

Specialty Biology

Date 2020 Mar 1

PMID 32111840

Citations 21

Authors

Colin W Hiebert

Matthew J Moscou

Tim Hewitt

Burkhard Steuernagel

Inma Hernandez-Pinzon

Phon Green

Vincent Pujol

Peng Zhang

Matthew N Rouse

Yue Jin

Robert A McIntosh

Narayana Upadhyaya

Jianping Zhang

Sridhar Bhavani

Jan Vrana

Miroslava Karafiatova

Li Huang

Tom Fetch

Jaroslav Dolezel

Brande B H Wulff

Evans Lagudah

Wolfgang Spielmeyer

Affiliations

Soon will be listed here.

Abstract

Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.

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