Wheat Pm55 Alleles Exhibit Distinct Interactions with an Inhibitor to Cause Different Powdery Mildew Resistance

Overview

Journal Nat Commun

Specialty Biology

Date 2024 Jan 13

PMID 38218848

Authors

Chuntian Lu

Jie Du

Heyu Chen

Shuangjun Gong

Yinyu Jin

Xiangru Meng

Ting Zhang

Bisheng Fu

Istvan Molnar

Katerina Holusova

Mahmoud Said

Liping Xing

Lingna Kong

Jaroslav Dolezel

Genying Li

Jizhong Wu

Peidu Chen

Aizhong Cao

Ruiqi Zhang

Affiliations

Soon will be listed here.

Abstract

Powdery mildew poses a significant threat to wheat crops worldwide, emphasizing the need for durable disease control strategies. The wheat-Dasypyrum villosum T5AL·5 V#4 S and T5DL·5 V#4 S translocation lines carrying powdery mildew resistant gene Pm55 shows developmental-stage and tissue-specific resistance, whereas T5DL·5 V#5 S line carrying Pm5V confers resistance at all stages. Here, we clone Pm55 and Pm5V, and reveal that they are allelic and renamed as Pm55a and Pm55b, respectively. The two Pm55 alleles encode coiled-coil, nucleotide-binding site-leucine-rich repeat (CNL) proteins, conferring broad-spectrum resistance to powdery mildew. However, they interact differently with a linked inhibitor gene, SuPm55 to cause different resistance to wheat powdery mildew. Notably, Pm55 and SuPm55 encode unrelated CNL proteins, and the inactivation of SuPm55 significantly reduces plant fitness. Combining SuPm55/Pm55a and Pm55b in wheat does not result in allele suppression or yield penalty. Our results provide not only insights into the suppression of resistance in wheat, but also a strategy for breeding durable resistance.

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