Discovery of Stripe Rust Resistance with Incomplete Dominance in Wild Emmer Wheat using Bulked Segregant Analysis Sequencing

Overview

Journal Commun Biol

Specialty Biology

Date 2022 Aug 17

PMID 35978056

Authors

Valentyna Klymiuk

Harmeet Singh Chawla

Krystalee Wiebe

Jennifer Ens

Andrii Fatiukha

Liubov Govta

Tzion Fahima

Curtis J Pozniak

Affiliations

Soon will be listed here.

Abstract

Durable crop disease resistance is an essential component of global food security. Continuous pathogen evolution leads to a breakdown of resistance and there is a pressing need to characterize new resistance genes for use in plant breeding. Here we identified an accession of wild emmer wheat (Triticum turgidum ssp. dicoccoides), PI 487260, that is highly resistant to multiple stripe rust isolates. Genetic analysis revealed resistance was conferred by a single, incompletely dominant gene designated as Yr84. Through bulked segregant analysis sequencing (BSA-Seq) we identified a 52.7 Mb resistance-associated interval on chromosome 1BS. Detected variants were used to design genetic markers for recombinant screening, further refining the interval of Yr84 to a 2.3-3.3 Mb in tetraploid wheat genomes. This interval contains 34 candidate genes encoding for protein domains involved in disease resistance responses. Furthermore, KASP markers closely-linked to Yr84 were developed to facilitate marker-assisted selection for rust resistance breeding.

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