Rapid Gene Isolation in Barley and Wheat by Mutant Chromosome Sequencing

Overview

Journal Genome Biol

Specialties Biology
Genetics

Date 2016 Nov 1

PMID 27795210

Citations 140

Authors

Javier Sanchez-Martin

Burkhard Steuernagel

Sreya Ghosh

Gerhard Herren

Severine Hurni

Nikolai Adamski

Jan Vrana

Marie Kubalakova

Simon G Krattinger

Thomas Wicker

Jaroslav Dolezel

Beat Keller

Brande B H Wulff

Affiliations

Soon will be listed here.

Abstract

Identification of causal mutations in barley and wheat is hampered by their large genomes and suppressed recombination. To overcome these obstacles, we have developed MutChromSeq, a complexity reduction approach based on flow sorting and sequencing of mutant chromosomes, to identify induced mutations by comparison to parental chromosomes. We apply MutChromSeq to six mutants each of the barley Eceriferum-q gene and the wheat Pm2 genes. This approach unambiguously identified single candidate genes that were verified by Sanger sequencing of additional mutants. MutChromSeq enables reference-free forward genetics in barley and wheat, thus opening up their pan-genomes to functional genomics.

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