Coumarins from As Inhibitors of the Tumour-associated Carbonic Anhydrases IX and XII: Isolation, Biological Studies and in Silico Evaluation

Overview

Journal J Enzyme Inhib Med Chem

Specialty Biochemistry

Date 2020 Jan 18

PMID 31948300

Citations 9

Authors

Benedetta Fois

Simona Distinto

Rita Meleddu

Serenella Deplano

Elias Maccioni

Costantino Floris

Antonella Rosa

Mariella Nieddu

Pierluigi Caboni

Claudia Sissi

Andrea Angeli

Claudiu T Supuran

Filippo Cottiglia

Affiliations

Soon will be listed here.

Abstract

In an screening for human carbonic anhydrase (hCA) inhibiting agents from higher plants, the petroleum ether and ethyl acetate extracts of seeds selectively inhibited hCA IX and hCA XII isoforms. The phytochemical investigation of the extracts led to the isolation of ten linear furocoumarins (-), four simple coumarins (-) and a new angular dihydrofurocoumarin (). The structures of the isolated compounds were elucidated based on 1 D and 2 D NMR, MS, and ECD data analysis. All isolated compounds were inactive towards the ubiquitous cytosolic isoform hCA I and II ( > 10,000 nM) while they were significantly active against the tumour-associated isoforms hCA IX and XII. Umbelliprenin was the most potent coumarin inhibiting hCA XII isoform with a of 5.7 nM. The cytotoxicity of the most interesting compounds on HeLa cancer cells was also investigated.

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