A Single-cell Atlas of the Developing Ovary Identifies Follicle Stem Cell Progenitors

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2020 Jan 11

PMID 31919193

Citations 40

Authors

Maija Slaidina

Torsten U Banisch

Selena Gupta

Ruth Lehmann

Affiliations

Soon will be listed here.

Abstract

Addressing the complexity of organogenesis at a system-wide level requires a complete understanding of adult cell types, their origin, and precursor relationships. The ovary has been a model to study how coordinated stem cell units, germline, and somatic follicle stem cells maintain and renew an organ. However, lack of cell type-specific tools have limited our ability to study the origin of individual cell types and stem cell units. Here, we used a single-cell RNA sequencing approach to uncover all known cell types of the developing ovary, reveal transcriptional signatures, and identify cell type-specific markers for lineage tracing. Our study identifies a novel cell type corresponding to the elusive follicle stem cell precursors and predicts subtypes of known cell types. Altogether, we reveal a previously unanticipated complexity of the developing ovary and provide a comprehensive resource for the systematic analysis of ovary morphogenesis.

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