A Cell Atlas of the Adult Female Aedes Aegypti Midgut Revealed by Single-cell RNA Sequencing

Overview

Journal Sci Data

Specialty Science

Date 2024 Jun 5

PMID 38839790

Authors

Shunlong Wang

Ying Huang

Fei Wang

Qian Han

Nanjie Ren

Xiaoyu Wang

Yingjun Cui

Zhiming Yuan

Han Xia

Affiliations

Soon will be listed here.

Abstract

Aedes aegypti is a primary vector for transmitting various arboviruses, including Yellow fever, dengue and Zika virus. The mosquito midgut is the principal organ for blood meal digestion, nutrient absorption and the initial site of arbovirus infection. Although a previous study delineated midgut's transcriptome of Ae. aegypti at the single-nucleus resolution, there still lacks an established protocol for isolating and RNA sequencing of single cells of Ae. aegypti midgut, which is required for investigating arbovirus-midgut interaction at the single-cell level. Here, we established an atlas of the midgut cells for Ae. aegypti by single-cell RNA sequencing. We annotated the cell clusters including intestinal stem cells/enteroblasts (ISC/EB), cardia cells (Cardia), enterocytes (EC, EC-like), enteroendocrine cells (EE), visceral muscle (VM), fat body cells (FBC) and hemocyte cells (HC). This study will provide a foundation for further studies of arbovirus infection in mosquito midgut at the single-cell level.

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