Genome Organization Regulates Nuclear Pore Complex Formation and Promotes Differentiation During Drosophila Oogenesis

Overview

Journal bioRxiv

Date 2023 Nov 28

PMID 38014330

Authors

Noor M Kotb

Gulay Ulukaya

Ankita Chavan

Son C Nguyen

Lydia Proskauer

Eric Joyce

Dan Hasson

Madhav Jagannathan

Prashanth Rangan

Affiliations

Soon will be listed here.

Abstract

Genome organization can regulate gene expression and promote cell fate transitions. The differentiation of germline stem cells (GSCs) to oocytes in Drosophila involves changes in genome organization mediated by heterochromatin and the nuclear pore complex (NPC). Heterochromatin represses germ-cell genes during differentiation and NPCs anchor these silenced genes to the nuclear periphery, maintaining silencing to allow for oocyte development. Surprisingly, we find that genome organization also contributes to NPC formation, mediated by the transcription factor Stonewall (Stwl). As GSCs differentiate, Stwl accumulates at boundaries between silenced and active gene compartments. Stwl at these boundaries plays a pivotal role in transitioning germ-cell genes into a silenced state and activating a group of oocyte genes and Nucleoporins (Nups). The upregulation of these Nups during differentiation is crucial for NPC formation and further genome organization. Thus, crosstalk between genome architecture and NPCs is essential for successful cell fate transitions.

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