Evidence That the Histamine Sensitivity and Responsiveness of Guinea-pig Isolated Trachea Are Modulated by Epithelial Prostaglandin E2 Production

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1988 Sep 1

PMID 3146396

Citations 15

Authors

G Braunstein

C Labat

S Brunelleschi

J Benveniste

J Marsac

C Brink

Affiliations

Soon will be listed here.

Abstract

1. Guinea-pig isolated tracheal preparations in which the epithelium had been removed exhibited a greater contractile response to histamine (intact: 1.91 +/- 0.12 g; n = 6 and rubbed: 2.76 +/- 0.15 g; n = 11; P less than 0.001). The histamine sensitivity (pD2 value) of these preparations was also significantly greater (intact: 4.80 +/- 0.04 and rubbed: 5.40 +/- 0.08; P less than 0.01). 2. Indomethacin suppressed the basal tone of both intact and rubbed preparations but was more effective in the former tissues (intact: -0.70 +/- 0.14 g; n = 22 and rubbed: -0.17 +/- 0.05 g; n = 12; P less than 0.02). 3. Arachidonic acid (AA; 10 microM) suppressed the basal tone of intact tissues but contracted such preparations following indomethacin treatment (1.7 microM; 30 min). However, in rubbed tissues AA (10 microM) induced a contraction which was attenuated following indomethacin treatment. 4. Prostaglandin E2 (PGE2; 0.01 and 0.1 microM) suppressed the basal tone of intact preparations and always evoked contraction of rubbed tissues. Following indomethacin treatment PGE2 (0.01 and 0.1 microM) generally evoked spasm of intact and rubbed tissues while at higher concentrations (1 microM) relaxant effects were observed. 5. Removal of the epithelium did not alter the relaxant effect of PGE2 (pD2 value) on histamine (50 microM)-contracted tissues (intact: 6.86 +/- 0.08 and rubbed: 7.10 +/- 0.3; n = 4; P greater than 0.1). 6. In rubbed preparations treated with indomethacin, PGE2 (0.01 and 0.1 microM) evoked spasm. However, when added to preparations contracted with 5 microM histamine, PGE2 always caused relaxation. 7. The release of immunoreactive PGE2 by rubbed preparations during histamine and/or AA stimulation was significantly less than that produced by intact stimulated tissues. 8. Exogenous PGE2 (0.01-1 microM) decreased the maximal response and sensitivity of rubbed tracheal preparations to histamine. 9. These results suggest that release of an epithelial derived cyclo-oxygenase product, namely PGE2, may regulate basal tone, histamine response and sensitivity of the guinea-pig isolated trachea.

Citing Articles

Calcium Signaling in Airway Epithelial Cells: Current Understanding and Implications for Inflammatory Airway Disease.

Jairaman A, Prakriya M Arterioscler Thromb Vasc Biol. 2024; 44(4):772-783.

PMID: 38385293 PMC: 11090472. DOI: 10.1161/ATVBAHA.123.318339.

New phosphodiesterase-4 inhibitors present airways relaxant activity in a guinea pig acute asthma model.

Martins I, de Melo Medeiros M, Vasconcelos L, Silva M, Queiroga F, Cavalcante-Silva L Naunyn Schmiedebergs Arch Pharmacol. 2023; 397(6):4419-4434.

PMID: 38108836 DOI: 10.1007/s00210-023-02905-8.

Epithelial Cells Induce a Cyclo-Oxygenase-1-Dependent Endogenous Reduction in Airway Smooth Muscle Contractile Phenotype.

OSullivan M, Gabriel E, Panariti A, Park C, Ijpma G, Fredberg J Am J Respir Cell Mol Biol. 2017; 57(6):683-691.

PMID: 28708434 PMC: 5765417. DOI: 10.1165/rcmb.2016-0427OC.

Interferon gamma induces prostaglandin G/H synthase-2 through an autocrine loop via the epidermal growth factor receptor in human bronchial epithelial cells.

Asano K, Nakamura H, Lilly C, Klagsbrun M, Drazen J J Clin Invest. 1997; 99(5):1057-63.

PMID: 9062364 PMC: 507914. DOI: 10.1172/JCI119233.

Bronchodilatation of guinea-pig perfused bronchioles induced by the H3-receptor for histamine: role of epithelium.

Burgaud J, Oudart N Br J Pharmacol. 1993; 109(4):960-6.

PMID: 8401948 PMC: 2175714. DOI: 10.1111/j.1476-5381.1993.tb13714.x.

References

Orehek J, Douglas J, Lewis A, Bouhuys A . Prostaglandin regulation of airway smooth muscle tone. Nat New Biol. 1973; 245(142):84-5. DOI: 10.1038/newbio245084a0. View

Tschirhart E, Frossard N, Bertrand C, Landry Y . Arachidonic acid metabolites and airway epithelium-dependent relaxant factor. J Pharmacol Exp Ther. 1987; 243(1):310-6. View

Orehek J, Douglas J, Bouhuys A . Contractile responses of the guinea-pig trachea in vitro: modification by prostaglandin synthesis-inhibiting drugs. J Pharmacol Exp Ther. 1975; 194(3):554-64. View

Gardiner P, COLLIER H . Specific receptors for prostaglandins in airways. Prostaglandins. 1980; 19(6):819-41. DOI: 10.1016/0090-6980(80)90116-1. View

Adcock J, Garland L . A possible role for lipoxygenase products as regulators of airway smooth muscle reactivity. Br J Pharmacol. 1980; 69(2):167-9. PMC: 2044254. DOI: 10.1111/j.1476-5381.1980.tb07886.x. View

Mitchell H, Denborough M . The metabolism of arachidonic acid in the isolated tracheal and lung strip preparations of guinea-pigs. Lung. 1980; 158(3):121-9. DOI: 10.1007/BF02713714. View

Flavahan N, Aarhus L, Rimele T, Vanhoutte P . Respiratory epithelium inhibits bronchial smooth muscle tone. J Appl Physiol (1985). 1985; 58(3):834-8. DOI: 10.1152/jappl.1985.58.3.834. View

Coleman R, Kennedy I . Characterisation of the prostanoid receptors mediating contraction of guinea-pig isolated trachea. Prostaglandins. 1985; 29(3):363-75. DOI: 10.1016/0090-6980(85)90096-6. View

Barnes P, Cuss F, Palmer J . The effect of airway epithelium on smooth muscle contractility in bovine trachea. Br J Pharmacol. 1985; 86(3):685-91. PMC: 1916714. DOI: 10.1111/j.1476-5381.1985.tb08946.x. View

10.

Gardiner P . Characterization of prostanoid relaxant/inhibitory receptors (psi) using a highly selective agonist, TR4979. Br J Pharmacol. 1986; 87(1):45-56. PMC: 1916908. DOI: 10.1111/j.1476-5381.1986.tb10155.x. View

11.

Goldie R, Papadimitriou J, Paterson J, Rigby P, Self H, Spina D . Influence of the epithelium on responsiveness of guinea-pig isolated trachea to contractile and relaxant agonists. Br J Pharmacol. 1986; 87(1):5-14. PMC: 1916890. DOI: 10.1111/j.1476-5381.1986.tb10150.x. View

12.

Hay D, FARMER S, Raeburn D, Robinson V, FLEMING W, Fedan J . Airway epithelium modulates the reactivity of guinea-pig respiratory smooth muscle. Eur J Pharmacol. 1986; 129(1-2):11-8. DOI: 10.1016/0014-2999(86)90330-4. View

13.

Cerrina J, Raffestin B, Labat C, Boullet C, Bayol A, Benveniste J . Histamine contraction of isolated human airway muscle preparations: role of prostaglandins. J Pharmacol Exp Ther. 1986; 239(2):536-41. View

14.

FARMER S, Fedan J, Hay D, Raeburn D . The effects of epithelium removal on the sensitivity of guinea-pig isolated trachealis to bronchodilator drugs. Br J Pharmacol. 1986; 89(2):407-14. PMC: 1917016. DOI: 10.1111/j.1476-5381.1986.tb10274.x. View

15.

Holroyde M . The influence of epithelium on the responsiveness of guinea-pig isolated trachea. Br J Pharmacol. 1986; 87(3):501-7. PMC: 1916580. DOI: 10.1111/j.1476-5381.1986.tb10192.x. View

16.

Ilhan M, Sahin I . Tracheal epithelium releases a vascular smooth muscle relaxant factor: demonstration by bioassay. Eur J Pharmacol. 1986; 131(2-3):293-6. DOI: 10.1016/0014-2999(86)90586-8. View

17.

Nijkamp F, Folkerts G . Reversal of arachidonic acid-induced guinea-pig tracheal relaxation into contraction after epithelium removal. Eur J Pharmacol. 1986; 131(2-3):315-6. DOI: 10.1016/0014-2999(86)90591-1. View

18.

BUTLER G, Adler K, EVANS J, Morgan D, Szarek J . Modulation of rabbit airway smooth muscle responsiveness by respiratory epithelium. Involvement of an inhibitory metabolite of arachidonic acid. Am Rev Respir Dis. 1987; 135(5):1099-104. DOI: 10.1164/arrd.1987.135.5.1099. View

19.

FARMER S, Hay D, Raeburn D, Fedan J . Relaxation of guinea-pig tracheal smooth muscle to arachidonate is converted to contraction following epithelium removal. Br J Pharmacol. 1987; 92(1):231-6. PMC: 1853639. DOI: 10.1111/j.1476-5381.1987.tb11316.x. View

20.

Grodzinska L, Panczenko B, Gryglewski R . Generation of prostaglandin E-like material by the guinea-pig trachea contracted by histamine. J Pharm Pharmacol. 1975; 27(2):88-91. DOI: 10.1111/j.2042-7158.1975.tb09414.x. View