Contractile Properties of Synthetic Cationic Polypeptides in Guinea-pig Isolated Trachea

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1994 Jan 1

PMID 8012709

Citations 6

Authors

D Spina

R G Goldie

Affiliations

Soon will be listed here.

Abstract

1. The synthetic polypeptides, poly-L-arginine, poly-L-lysine and poly-D-lysine contract guinea-pig isolated trachea in a concentration-dependent, epithelium-independent manner. Indomethacin augmented the contractile response to poly-L-arginine. 2. The contractile response to poly-L-arginine was not significantly inhibited by nicardipine, a selective L-type calcium channel blocker or by the histamine H1-receptor antagonist, mepyramine nor significantly augmented by the neutral endopeptidase inhibitor, phosphoramidon. 3. The contractile response to poly-L-arginine was inhibited in a concentration-dependent manner by prior incubation of guinea-pig tracheal rings with a number of anionic polypeptides including, low molecular weight heparin, poly-L-aspartic acid and bovine serum albumin. 4. In vitro capsaicin-induced desensitization failed to attenuate the contractile response to poly-L-arginine, suggesting little, if any role for sensory neuropeptides in the functional response in the guinea-pig. 5. Synthetic polypeptides induce an epithelium-independent, charge-dependent contraction of guinea-pig isolated trachea.

Citing Articles

Poly-L-Arginine Induces Apoptosis of NCI-H292 Cells via ERK1/2 Signaling Pathway.

Wang Y, Zhang L, Fan X, Wu S, Zhang S J Immunol Res. 2018; 2018:3651743.

PMID: 30013990 PMC: 6022307. DOI: 10.1155/2018/3651743.

Effects of the cationic protein poly-L-arginine on airway epithelial cells in vitro.

Shahana S, Kampf C, Roomans G Mediators Inflamm. 2002; 11(3):141-8.

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Selective inflammatory response induced by intratracheal and intravenous administration of poly-L-arginine in guinea pig lungs.

Arseneault D, Maghni K, Sirois P Inflammation. 1999; 23(3):287-304.

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Granulocyte recruitment in guinea pig lungs following the administration of MBP-like cationic protein.

Arseneault D, Maghni K, Sirois P Inflamm Res. 1995; 44 Suppl 2:S166-7.

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Poly-L-arginine-mediated release of acetylcholine from parasympathetic nerves in rat and guinea-pig airways.

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PMID: 7921618 PMC: 1910221. DOI: 10.1111/j.1476-5381.1994.tb13164.x.

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