The Effect of Airway Epithelium on Smooth Muscle Contractility in Bovine Trachea

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1985 Nov 1

PMID 4063586

Citations 64

Authors

P J Barnes

F M Cuss

J B Palmer

Affiliations

Soon will be listed here.

Abstract

In bovine tracheal smooth muscle the presence of airway epithelium significantly reduced the sensitivity and maximum contractile response to histamine, 5-hydroxytryptamine (5-HT) or acetylcholine. Muscle contraction induced by K+ and electrical field stimulation was of similar magnitude both in the presence or absence of adherent epithelium. The effect of epithelium on smooth muscle contractility was unaffected by pretreatment with indomethacin (10(-6) M) or mepacrine (5 X 10(-5) M). The relaxant response to isoprenaline was enhanced in the presence of epithelium, although this was significant only in the case of precontraction with 5-HT. It is concluded that the bronchial epithelium may produce a relaxant factor which is not a cyclooxygenase or lipoxygenase product. The production of this factor may be reduced or lost following epithelial damage and this may be important in the pathogenesis of bronchial hyperresponsiveness in asthma.

Citing Articles

5-HT, 5-HT, 5HT and 5-HT receptors as mediators of serotonin-induced direct contractile response of bovine airway smooth muscle.

Da Costa Guevara D, Trejo E J Smooth Muscle Res. 2022; 57(0):79-93.

PMID: 34980821 PMC: 8710915. DOI: 10.1540/jsmr.57.79.

Integrin α2β1 regulates collagen I tethering to modulate hyperresponsiveness in reactive airway disease models.

Liu S, Ngo U, Tang X, Ren X, Qiu W, Huang X J Clin Invest. 2021; 131(12).

PMID: 33956668 PMC: 8203456. DOI: 10.1172/JCI138140.

Butyrlycholine esterase inhibitory activity and effects of extracts (fruit, bark and leaf) from Zanthoxylum armatum DC in gut, airways and vascular smooth muscles.

Alam F, Shah A BMC Complement Altern Med. 2019; 19(1):180.

PMID: 31331314 PMC: 6647253. DOI: 10.1186/s12906-019-2597-2.

Ezrin, a Membrane Cytoskeleton Cross-Linker Protein, as a Marker of Epithelial Damage in Asthma.

Jia M, Yan X, Jiang X, Wu Y, Xu J, Meng Y Am J Respir Crit Care Med. 2018; 199(4):496-507.

PMID: 30290132 PMC: 6376623. DOI: 10.1164/rccm.201802-0373OC.

Gene expression analysis in asthma using a targeted multiplex array.

Pascoe C, Obeidat M, Arsenault B, Nie Y, Warner S, Stefanowicz D BMC Pulm Med. 2017; 17(1):189.

PMID: 29228930 PMC: 5725935. DOI: 10.1186/s12890-017-0545-9.

References

Empey D, Laitinen L, Jacobs L, Gold W, Nadel J . Mechanisms of bronchial hyperreactivity in normal subjects after upper respiratory tract infection. Am Rev Respir Dis. 1976; 113(2):131-9. DOI: 10.1164/arrd.1976.113.2.131. View

Dunnill M . The pathology of asthma, with special reference to changes in the bronchial mucosa. J Clin Pathol. 1960; 13:27-33. PMC: 479992. DOI: 10.1136/jcp.13.1.27. View

Frigas E, Loegering D, Gleich G . Cytotoxic effects of the guinea pig eosinophil major basic protein on tracheal epithelium. Lab Invest. 1980; 42(1):35-43. View

Frigas E, Loegering D, Solley G, Farrow G, Gleich G . Elevated levels of the eosinophil granule major basic protein in the sputum of patients with bronchial asthma. Mayo Clin Proc. 1981; 56(6):345-53. View

Filley W, HOLLEY K, Kephart G, Gleich G . Identification by immunofluorescence of eosinophil granule major basic protein in lung tissues of patients with bronchial asthma. Lancet. 1982; 2(8288):11-6. DOI: 10.1016/s0140-6736(82)91152-7. View

Holtzman M, Aizawa H, Nadel J, Goetzl E . Selective generation of leukotriene B4 by tracheal epithelial cells from dogs. Biochem Biophys Res Commun. 1983; 114(3):1071-6. DOI: 10.1016/0006-291x(83)90671-x. View

Griffith T, Edwards D, Lewis M, Newby A, Henderson A . The nature of endothelium-derived vascular relaxant factor. Nature. 1984; 308(5960):645-7. DOI: 10.1038/308645a0. View

FURCHGOTT R . The role of endothelium in the responses of vascular smooth muscle to drugs. Annu Rev Pharmacol Toxicol. 1984; 24:175-97. DOI: 10.1146/annurev.pa.24.040184.001135. View

Woolcock A, Salome C, Yan K . The shape of the dose-response curve to histamine in asthmatic and normal subjects. Am Rev Respir Dis. 1984; 130(1):71-5. DOI: 10.1164/arrd.1984.130.1.71. View

10.

Boushey H, Holtzman M . Experimental airway inflammation and hyperreactivity. Searching for cells and mediators. Am Rev Respir Dis. 1985; 131(3):312-3. DOI: 10.1164/arrd.1985.131.3.312. View

11.

Murlas C, Roum J . Sequence of pathologic changes in the airway mucosa of guinea pigs during ozone-induced bronchial hyperreactivity. Am Rev Respir Dis. 1985; 131(3):314-20. DOI: 10.1164/arrd.1985.131.3.314. View

12.

Laitinen L, Heino M, Laitinen A, Kava T, Haahtela T . Damage of the airway epithelium and bronchial reactivity in patients with asthma. Am Rev Respir Dis. 1985; 131(4):599-606. DOI: 10.1164/arrd.1985.131.4.599. View

13.

Sanerkin N, Evans D . THE SPUTUM IN BRONCHIAL ASTHMA: PATHOGNOMONIC PATTERNS. J Pathol Bacteriol. 1965; 89:535-41. View

14.

Naylor B . The shedding of the mucosa of the bronchial tree in asthma. Thorax. 1962; 17:69-72. PMC: 1018672. DOI: 10.1136/thx.17.1.69. View

15.

Cutz E, Levison H, Cooper D . Ultrastructure of airways in children with asthma. Histopathology. 1978; 2(6):407-21. DOI: 10.1111/j.1365-2559.1978.tb01735.x. View